Tissue homogenates serve as a model system for understanding physiological processes and screening of toxic or therapeutic compounds for use in medical treatments. There are tissue homogenate protocols available for Biovendor's immunoassays or can be developed and verified by users themselves for specific applications. The protocols vary in a tissue homogenization step or composition of extraction buffers, often containing both ionic and non-ionic detergents and protease inhibitors, in dependence on the type of tissue being processed.