Type
Sandwich ELISA, HRP-labelled antibody
Description
The Urine BETA CrossLaps® (CTX-I) ELISA is an enzyme immunological test for the quantification of degradation products of C-terminal telopeptides of Type-I collagen in human urine. The Urine BETA CrossLaps® (CTX-I) ELISA assay is intended for in vitro diagnostic use as an indication of human bone resorption as an aid in:
- Monitoring bone resorption changes of anti-resorptive therapies in postmenopausal women:
- Hormone Replacement Therapies (HRT) with hormones and hormone-like drugs
- Bisphosphonate therapies
- Predicting skeletal response (Bone Mineral Density) in postmenopausal women undergoing anti-resorptive therapies
- Hormone Replacement Therapies (HRT) with hormones and hormone-like drugs
- Bisphosphonate therapies
Scientific Description:
Type I collagen accounts for more than 90% of the organic matrix of bone and is synthesised primarily in bone. During renewal of the skeleton, type I collagen is degraded, and small peptide fragments are excreted into the urine. These fragments can be measured by the Urine BETA CrossLaps® (CTX-I) ELISA. The Urine BETA CrossLaps® (CTX-I) ELISA is based on two highly specific monoclonal antibodies against the amino acid sequence of EKAHD-β-GGR, where the aspartic acid residue (D) is β-isomerized. In order to obtain a specific signal in the Urine BETA CrossLaps®(CTX-I) ELISA, two chains of EKAHD-β-GGR must be cross-linked. Urine BETA CrossLaps® (CTX-I) ELISA demonstrates a high correlation to corresponding measurements of serum samples in the Serum CrossLaps® (CTX-I) ELISA.
Applications
Urine
Sample Requirements
10 μL Pre-diluted (1+3)
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
0-40.7 μg/L
Limit of Detection
0.80 μg/L CrossLaps
Note
The kits are CE-IVD certified and intended for professional use.