sTfR Human ELISA (soluble Transferrin Receptor)

Cat. No.	Size	Price
RD194011100	96 wells (1 kit)	$457

Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.05–2 µg/ml

Limit of Detection

2 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 6.8%

Inter-assay (Run-to-Run)

n = 4; CV = 6.3%

Spiking Recovery

100,20%

Dilution Linearity

94,00%

Crossreactivity

bovine Non-detectable
cat Non-detectable
dog Non-detectable
hamster Non-detectable
horse Non-detectable
goat Yes
mouse Non-detectable
rabbit Non-detectable
rat Non-detectable
sheep Non-detectable
chicken Not tested
monkey Yes
pig Yes
human Yes

Note

The kits are CE-IVD certified and intended for professional use.

Summary

Features

European Union: for in vitro diagnostic use
Rest of the world: for research use only!
The total assay time is less than 3 hours
The kit measures total soluble transferrin receptor in serum and plasma (EDTA, citrate, heparin)
Assay format is 96 wells
Quality Controls are human serum based. No animal sera are used
Standard is natural human blood isolated sTfR based
Components of the kit are provided ready to use or concentrated

Research topic

Iron metabolism, Oncology

Summary

The transferrin receptor (TfR) is the gateway for transferrin-bound-iron entering all body cells. TfR is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body TfR content. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The serum sTfR concentration reflects the total body mass of cellular transferrin receptor. Anaemias associated with enhanced erythropoiesis and iron deficiency result in an elevation in the sTfR values. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in its decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

Product References (23)

References

Barth-Jaeggi T, Zandberg L, Bahruddinov M, Kiefer S, Rahmarulloev S, Wyss K. Nutritional status of Tajik children and women: Transition towards a double burden of malnutrition. Matern Child Nutr. 2020 Apr;16(2):e12886. doi: 10.1111/mcn.12886. Epub 2019 Nov 8. PubMed PMID: 31702104. PubMed CentralPMCID: PMC7083412. See more on PubMed
Muriuki JM, Mentzer AJ, Kimita W, Ndungu FM, Macharia AW, Webb EL, Lule SA, Morovat A, Hill AVS, Bejon P, Elliott AM, Williams TN, Atkinson SH. Iron Status and Associated Malaria Risk Among African Children. Clin Infect Dis. 2019 May 17;68(11):1807-1814. doi: 10.1093/cid/ciy791. PubMed PMID: 30219845. PubMed CentralPMCID: PMC6522755. See more on PubMed
Fukao W, Hasuike Y, Yamakawa T, Toyoda K, Aichi M, Masachika S, Kantou M, Takahishi SI, Iwasaki T, Yahiro M, Nanami M, Nagasawa Y, Kuragano T, Nakanishi T. Oral Versus Intravenous Iron Supplementation for the Treatment of Iron Deficiency Anemia in Patients on Maintenance Hemodialysis-Effect on Fibroblast Growth Factor-23 Metabolism. J Ren Nutr. 2018 Jul;28(4):270-277. doi: 10.1053/j.jrn.2017.12.009. Epub 2018 Apr 24. PubMed PMID: 29703633. See more on PubMed
Loy SL, Lim LM, Chan SY, Tan PT, Chee YL, Quah PL, Chan JKY, Tan KH, Yap F, Godfrey KM, Shek LP, Chong MF, Kramer MS, Chong YS, Chi C. Iron status and risk factors of iron deficiency among pregnant women in Singapore: a cross-sectional study. BMC Public Health. 2019 Apr 11;19(1):397. doi: 10.1186/s12889-019-6736-y. PubMed PMID: 30975203. PubMed CentralPMCID: PMC6460529. See more on PubMed
Tiwari AK, Bhardwaj G, Arora D, Aggarwal G, Pabbi S, Dara RC, Sachdev R, Raizada A, Sethi M. Applying newer parameter Ret-He (reticulocyte haemoglobin equivalent) to assess latent iron deficiency (LID) in blood donors-study at a tertiary care hospital in India. Vox Sang. 2018 Oct;113(7):639-646. doi: 10.1111/vox.12700. Epub 2018 Aug 12. PubMed PMID: 30101488. See more on PubMed
Brzóska K, Bartłomiejczyk T, Sochanowicz B, Cymerman M, Grudny J, Kołakowski J, Kruszewski M, Śliwiński P, Roszkowski-Śliż K, Kapka-Skrzypczak L. Carcinogenesis-related changes in iron metabolism in chronic obstructive pulmonary disease subjects with lung cancer. Oncol Lett. 2018 Nov;16(5):6831-6837. doi: 10.3892/ol.2018.9459. Epub 2018 Sep 19. PubMed PMID: 30405827. PubMed CentralPMCID: PMC6202463. See more on PubMed
Boshuizen M, Binnekade JM, Nota B, van de Groep K, Cremer OL, Tuinman PR, Horn J, Schultz MJ, van Bruggen R, Juffermans NP. Iron metabolism in critically ill patients developing anemia of inflammation: a case control study. Ann Intensive Care. 2018 May 2;8(1):56. doi: 10.1186/s13613-018-0407-5. PubMed PMID: 29717382. PubMed CentralPMCID: PMC5930297. See more on PubMed
Büyükkaragöz B, Akgun NA, Bulus AD, Durmus Aydogdu S, Bal C. Can soluble transferrin receptor be used in diagnosing iron deficiency anemia and assessing iron response in infants with moderate acute malnutrition?. Arch Argent Pediatr. 2017 Apr 1;115(2):125-132. doi: 10.5546/aap.2017.eng.125. PubMed PMID: 28318177. See more on PubMed
El-Gendy FM, El-Hawy MA, Rizk MS, El-Hefnawy SM, Mahmoud MZ. Value of Soluble Transferrin Receptors and sTfR/log Ferritin in the Diagnosis of Iron Deficiency Accompanied by Acute Infection. Indian J Hematol Blood Transfus. 2018 Jan;34(1):104-109. doi: 10.1007/s12288-017-0836-6. Epub 2017 May 25. PubMed PMID: 29398807. PubMed CentralPMCID: PMC5786629. See more on PubMed
Skarpańska-Stejnborn A, Basta P, Trzeciak J, Michalska A, Kafkas ME, Woitas-Ślubowska D. Effects of cranberry (Vaccinum macrocarpon) supplementation on iron status and inflammatory markers in rowers. J Int Soc Sports Nutr. 2017 Feb 28;14:7. doi: 10.1186/s12970-017-0165-z. eCollection 2017. PubMed PMID: 28261001. PubMed CentralPMCID: PMC5330006. See more on PubMed
Wenger MJ, Murray-Kolb LE, Nevins JE, Venkatramanan S, Reinhart GA, Wesley A, Haas JD. Consumption of a Double-Fortified Salt Affects Perceptual, Attentional, and Mnemonic Functioning in Women in a Randomized Controlled Trial in India. J Nutr. 2017 Dec;147(12):2297-2308. doi: 10.3945/jn.117.251587. Epub 2017 Oct 11. PubMed PMID: 29021371. PubMed CentralPMCID: PMC6519426. See more on PubMed
Dierschke K, Isaxon C, Andersson UBK, Assarsson E, Axmon A, Stockfelt L, Gudmundsson A, Jönsson BAG, Kåredal M, Löndahl J, Pagels J, Wierzbicka A, Bohgard M, Nielsen J. Acute respiratory effects and biomarkers of inflammation due to welding-derived nanoparticle aggregates. Int Arch Occup Environ Health. 2017 Jul;90(5):451-463. doi: 10.1007/s00420-017-1209-z. Epub 2017 Mar 3. PubMed PMID: 28258373. PubMed CentralPMCID: PMC5486570. See more on PubMed
Stefanova KI, Delcheva GT, Maneva AI, Batalov AZ, Geneva-Popova MG, Karalilova RV, Simitchiev KK. Pathobiochemical Mechanisms Relating Iron Homeostasis to Parameters of Inflammatory Activity and Autoimmune Disorders in Rheumatoid Arthritis. Folia Med (Plovdiv). 2016 Dec 1;58(4):257-263. doi: 10.1515/folmed-2016-0040. PubMed PMID: 28068286. See more on PubMed
Sharma JB, Bumma SD, Saxena R, Kumar S, Roy KK, Singh N, Vanamail P. Cross sectional, comparative study of serum erythropoietin, transferrin receptor, ferritin levels and other hematological indices in normal pregnancies and iron deficiency anemia during pregnancy. Eur J Obstet Gynecol Reprod Biol. 2016 Aug;203:99-103. doi: 10.1016/j.ejogrb.2016.05.022. Epub 2016 May 20. PubMed PMID: 27267870. See more on PubMed
Menon KC, Ferguson EL, Thomson CD, Gray AR, Zodpey S, Saraf A, Das PK, Pandav CS, Skeaff SA. Iron status of pregnant Indian women from an area of active iron supplementation. Nutrition. 2014 Mar;30(3):291-6. doi: 10.1016/j.nut.2013.08.015. PubMed PMID: 24484679. See more on PubMed
Jafari SM, Heidari G, Nabipour I, Amirinejad R, Assadi M, Bargahi A, Akbarzadeh S, Tahmasebi R, Sanjdideh Z. Serum retinol levels are positively correlated with hemoglobin concentrations, independent of iron homeostasis: a population-based study. Nutr Res. 2013 Apr;33(4):279-85. doi: 10.1016/j.nutres.2013.02.004. Epub 2013 Mar 11. PubMed PMID: 23602245. See more on PubMed
Rybka J, Kędziora-Kornatowska K, Banaś-Leżańska P, Majsterek I, Carvalho LA, Cattaneo A, Anacker C, Kędziora J. Interplay between the pro-oxidant and antioxidant systems and proinflammatory cytokine levels, in relation to iron metabolism and the erythron in depression. Free Radic Biol Med. 2013 Oct;63:187-94. doi: 10.1016/j.freeradbiomed.2013.05.019. Epub 2013 May 23. PubMed PMID: 23707456. See more on PubMed
Fernández-Real JM, Izquierdo M, Moreno-Navarrete JM, Gorostiaga E, Ortega F, Martínez C, Idoate F, Ricart W, Ibañez J. Circulating soluble transferrin receptor concentration decreases after exercise-induced improvement of insulin sensitivity in obese individuals. Int J Obes (Lond). 2009 Jul;33(7):768-74. doi: 10.1038/ijo.2009.99. Epub 2009 Jun 2. PubMed PMID: 19488049. See more on PubMed
Khambalia A, O'Connor DL, Zlotkin S. Periconceptional iron and folate status is inadequate among married, nulliparous women in rural Bangladesh. J Nutr. 2009 Jun;139(6):1179-84. doi: 10.3945/jn.108.101022. Epub 2009 Apr 29. PubMed PMID: 19403710. See more on PubMed
Mojiminiyi OA, Marouf R, Abdella NA. Body iron stores in relation to the metabolic syndrome, glycemic control and complications in female patients with type 2 diabetes. Nutr Metab Cardiovasc Dis. 2008 Oct;18(8):559-66. doi: 10.1016/j.numecd.2007.07.007. Epub 2007 Dec 11. PubMed PMID: 18063352. See more on PubMed
Shinzato T, Abe K, Furusu A, Harada T, Shinzato K, Miyazaki M, Kohno S. Serum pro-hepcidin level and iron homeostasis in Japanese dialysis patients with erythropoietin (EPO)-resistant anemia. Med Sci Monit. 2008 Sep;14(9):CR431-7. PubMed PMID: 18758412. See more on PubMed
Fernández-Real JM, Moreno JM, López-Bermejo A, Chico B, Vendrell J, Ricart W. Circulating soluble transferrin receptor according to glucose tolerance status and insulin sensitivity. Diabetes Care. 2007 Mar;30(3):604-8. doi: 10.2337/dc06-1138. PubMed PMID: 17327328. See more on PubMed
Mier PD, van den Hurk JJ. Lysosomal hydrolases of the epidermis. 2. Ester hydrolases. Br J Dermatol. 1975 Oct;93(4):391-8. doi: 10.1111/j.1365-2133.1975.tb06512.x. PubMed PMID: 31. See more on PubMed

Summary References (9)

References to Transferrin receptor

Raya G. et al.: Soluble transferrin receptor (sTfR): biological variations and reference limits. Clinical Chemistry and Laboratory Medicine, 39, 1162–1168 (2001)
Cotton F. et al.: Measurement of soluble transferrin receptor by immunoturbidimety and immunonefelometry. Clinical Biochemistry, 33, 263–267 (2000)
Cook J. D.: The measurement of serum transferrin receptor. The American Journal of the Medical Sciences 318, 269–276 (1999)
Olivares M. et al.: Usefulness of serum transferrin receptor and serum ferritin in diagnosis of iron deficiency in infancy. American Journal of Clinical Nutrition 72, 1191–1195 (2000)
Suominen P. et al.: Single values of serum transferrin receptor and transferrin receptor ferritin index can be used to detect true and functional iron deficiency in rheumatoid arthritis patients with anemia. Arthritis & Rheumatism 43, 1016–1020 (2000)
De Block C. E. M. et al.: Soluble transferrin receptor level. A new marker for iron deficiency anemia, a common manifestation of gastric autoimmunity in type 1diabetes. Diabetes Care 23, 1384–1388 (2000)
Kolbe-Busch S. et al. Determination of the soluble transferrin receptor in Serum: Evaluation of two enzyme immunoassays and a particle-enhanced immunonephelometric assay. Clinical Laborators 45, 295–304 (1999)
Hikawa A. et al.: Soluble transferrin receptor-transferrin complex in serum: measurement by latex agglutination nephelometric asssay. Clinica Chimica Acta 254, 159–172 (1996)
Flowers C. H. et al.: The clinical measurement of serum transferrin receptor. Journal of Laboratory and Clinical Medicine 114, 368–377 (1989)

By Research Topic

Iron metabolism
Oncology

By Molecule

Transferrin receptor

By Technical Data

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