Type
Sandwich ELISA, HRP-labelled antibody
Description
The Human sIL-2R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of Human sIL-2R. The Human sIL-2R ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
50 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.31–20 ng/ml
Limit of Detection
0.27 ng/ml
Intra-assay (Within-Run)
CV = 7.2%
Inter-assay (Run-to-Run)
CV = 9.8%
Spiking Recovery
75.0 %
Dilution Linearity
86,0%
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a Human IL-2R positive serum.
There was no crossreactivity detected.
Features
- RUO
- calibration range 0.31-20 ng/ml
- limit of detection 0.27 ng/ml
- intra-assay CV = 7.2%
- inter-assay CV = 9.8%
Research topic
Cytokines and chemokines and related molecules, Oncology, COVID-19
Summary
Interleukin-2 (IL-2) has been described as a factor that promotes the growth and proliferation of Human T-cells thus occupying a pivotal role in the generation of the immune response. This proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific cell surface receptor following T lymphocyte activation. The receptor for IL-2 is composed of at least three distinct polypeptide subunits, the IL-2R, IL-2R, and the IL-2R chains giving rise to a 55-65 kD membrane bound protein.
The genes encoding IL-2 and these three receptor subunits have been cloned and their complete primary structures have been deduced. Evidence has accumulated that suggests a critical role of IL-2R in IL-2 signal transduction. IL-2R coupled tyrosine kinases are believed to play a crucial role in that signal transduction.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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