Human sICAM-1 ELISA

Cat. No.	Size	Price
RAF102R	96 wells (1 kit)

Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Description

The human sICAM-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sICAM-1 The human sICAM-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Applications

Serum, Urine, Amniotic fluid, Plasma, Cell culture supernatant, Bile

Sample Requirements

10 µl/well

Shipping

On blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store kit reagents between 2–8°C except control. Store lyophilized control at -20°C.

Calibration Curve

Calibration Range

6.3–100 ng/ml

Limit of Detection

2.2 ng/ml

Intra-assay (Within-Run)

CV = 4.1%

Inter-assay (Run-to-Run)

CV = 7.7%

Spiking Recovery

99%.

Dilution Linearity

93%

Specificity

The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human sICAM-1 positive serum.There was no cross reactivity detected with sTNF-R (60 kDa), sTNF-R (80 kDa), IL-8/NAP-1, TNF, TNF, IFN, IFN2C, IFNω, IL-6, IL-2R, ELAM-1 and L-selectin.

Summary

Features

RUO
calibration range 6.3-100 ng/ml
limit of detection 2.2 ng/ml
intra-assay CV = 4.1%
inter-assay CV = 7.7%
lyophilized controls

Research topic

Cell adhesion proteins

Summary

Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-beta-complex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors. This family consists of the leukocyte adhesion glycoproteins LFA-1 which mediates lymphocyte adhesion, Mac-1 which mediates granulocyte adhesion and p150,95. ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR. The role of ICAM-1 as a disease marker has been demonstrated for a number of different indications and pathological situations.

Summary References (19)

References to ICAM-1

Adams DH, Hubscher SG, Shaw J, Rothlein R, Neuberger JM. Intercellular adhesion molecule 1 on liver allografts during rejection. Lancet. 1989 Nov 11;2 (8672):1122-5
Adams DH, Mainolfi E, Elias E, Neuberger JM, Rothlein R. Detection of circulating intercellular adhesion molecule-1 after liver transplantation--evidence of local release within the liver during graft rejection. Transplantation. 1993 Jan;55 (1):83-7
Altomonte M, Colizzi F, Esposito G, Maio M. Circulating intercellular adhesion molecule 1 as a marker of disease progression in cutaneous melanoma. N Engl J Med. 1992 Sep 24;327 (13):959
Arocker-Mettinger E, Steurer-Georgiew L, Steurer M, Huber-Spitzy V, Hoelzl E, Grabner G, Kuchar A. Circulating ICAM-1 levels in serum of uveitis patients. Curr Eye Res. 1992;11 Suppl:161-6
Becker JC, Dummer R, Schwinn A, Hartmann AA, Burg G. Circulating intercellular adhesion molecule-1 in melanoma patients: induction by interleukin-2 therapy. J Immunother (1991). 1992 Aug;12 (2):147-50
Dustin ML, Rothlein R, Bhan AK, Dinarello CA, Springer TA. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J. Immunol. 1986. 137: 245-254. J Immunol. 2011 May 1;186 (9):5024-33
Harning R, Mainolfi E, Bystryn JC, Henn M, Merluzzi VJ, Rothlein R. Serum levels of circulating intercellular adhesion molecule 1 in human malignant melanoma. Cancer Res. 1991 Sep 15;51 (18):5003-5
Lal RB, Mainolfi E, Rothlein R. Elevated levels of cICAM-1 in patients with human T-cell leukemia virus type I associated myelopathy and adult T-cell leukemia. Blood. 1992 Nov 1;80 (9):2434-5
Lampeter ER, Kishimoto TK, Rothlein R, Mainolfi EA, Bertrams J, Kolb H, Martin S. Elevated levels of circulating adhesion molecules in IDDM patients and in subjects at risk for IDDM. Diabetes. 1992 Dec;41 (12):1668-71
Lisby S, Ralfkiaer E, Rothlein R, Vejlsgaard GL. Intercellular adhesion molecule-I (ICAM-I) expression correlated to inflammation. Br J Dermatol. 1989 Apr;120 (4):479-84
Marlin SD, Springer TA. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell. 1987 Dec 4;51 (5):813-9
Most J, Zangerle R, Herold M, Fuchs D, Wachter H, Fritsch P, Dierich MP. Elevated concentrations of circulating intercellular adhesion molecule 1 (ICAM-1) in HIV-1 infection. J Acquir Immune Defic Syndr. 1993 Mar;6 (3):221-6
Pober JS, Gimbrone MA Jr, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. J Immunol. 1986 Sep 15;137 (6):1893-6
Potocnik AJ, Kinne R, Menninger H, Zacher J, Emmrich F, Kroczek RA. Expression of activation antigens on T cells in rheumatoid arthritis patients. Scand J Immunol. 1990 Feb;31 (2):213-24
Rothlein R, Czajkowski M, O'Neill MM, Marlin SD, Mainolfi E, Merluzzi VJ. Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies. J Immunol. 1988 Sep 1;141 (5):1665-9
Rothlein R, Dustin ML, Marlin SD, Springer TA. A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J. Immunol. 1986. 137: 1270-1274. J Immunol. 2011 May 1;186 (9):5034-8
Rothlein R, Mainolfi EA, Czajkowski M, Marlin SD. A form of circulating ICAM-1 in human serum. J Immunol. 1991 Dec 1;147 (11):3788-93
Salafia CM, DeVore GR, Mainolfi E, Kelly J, Pezzullo JC, Rothlein R. Circulating intercellular adhesion molecule-1 in amniotic fluid, maternal serum alpha-fetoprotein levels, and intrauterine growth retardation. Am J Obstet Gynecol. 1993 Oct;169 (4):830-4
Vejlsgaard GL, Ralfkiaer E, Avnstorp C, Czajkowski M, Marlin SD, Rothlein R. Kinetics and characterization of intercellular adhesion molecule-1 (ICAM-1) expression on keratinocytes in various inflammatory skin les

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