sCD44var (v6) Human ELISA

Cat. No.	Size	Price
RAF097R	96 wells (1 kit)	$721,83

Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Urine, Amniotic fluid, Cell culture supernatant, Body fluids

Sample Requirements

20 µl (1:20 prediluted)

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.32–10 ng/ml

Limit of Detection

0.05 ng/ml

Intra-assay (Within-Run)

CV = 3.0%

Inter-assay (Run-to-Run)

CV = 4.2%

Spiking Recovery

105,00%

Dilution Linearity

102,00%

Summary

Research topic

Cell adhesion proteins, Cell surface proteins (sCD), Extracellular matrix, Oncology

Summary

CD44 (Pgp-1; Ly-24; ECMR III; F10-44-2; H-CAM; HUTCH-I; In(Lu)-related p80; Hermes antigen; hyaluronan receptor) is a polymorphic glycoprotein which participates in a wide variety of cell-cell or cell-matrix interactions including lymphocyte homing, establishment of B and T cell immune responses, tumor metastasis formation and inflammation.
Three isoform categories of the CD44 molecule have been identified:

a predominant 80-90 kDa category, the so-called standard form named C44std,
an intermediate size category of 110-160 kDa and
a category which includes very large isoforms of 250 kDa covalently modified by the addition of chondroitin sulfate.
This CD44-family of transmembrane receptor molecules is derived from a single gene located on chromosome 11. Alternative splicing of the mRNA gives rise to the different isoforms, containing inserts of varying sizes in the extracellular domain of the molecule (exons v2-v10). All CD44 isoforms are variably glycosylated. In contrast to standard CD44 (CD44std) which is almost ubiquitously expressed (18), the variety of CD44 isoforms (CD44var) have a much more restricted distribution, e.g., on keratinocytes (exons v3-v10), ephitelial cells (exons v8-v10), activated lymphocytes and macrophages (exon v6).
A splice variant of CD44 (exons v4-v7) confers metastatic behaviour in a rat carcinoma model; aberrant expression of splice variants has been detected on a variety of human tumor cell lines as well as primary and metastatic human tumors, including lymphomas, carcinomas (colon, thyroid, mamma, bladder), and gliomas. Detection of abnormal regulation of CD44 splicing thus could be helpful in cancer diagnosis and disease evaluation. The sCD44var(v6) ELISA detects all circulating CD44 isoforms comprising the sCD44var(v6) sequences.

Summary References (17)

References to CD44

Arch R, Wirth K, Hofmann M, Ponta H, Matzku S, Herrlich P, Zoller M. Participation in normal immune responses of a metastasis-inducing splice variant of CD44. Science. 1992 Jul 31;257 (5070):682-5
Fujiwara K, Yoshino T, Miyake K, Ohara N, Akagi T. Expression of a lymphocyte adhesion molecule (CD44) in malignant lymphomas: relevance to primary site, histological subtype and clinical stage. Acta Med Okayama. 1993 Jun;47 (3):215-22
Gunthert U, Hofmann M, Rudy W, Reber S, Zoller M, Haussmann I, Matzku S, Wenzel A, Ponta H, Herrlich P. A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell. 1991 Apr 5;65 (1):13-24
Guo MM, Hildreth JE. HIV-induced loss of CD44 expression in monocytic cell lines. J Immunol. 1993 Aug 15;151 (4):2225-36
Heider KH, Dammrich J, Skroch-Angel P, Muller-Hermelink HK, Vollmers HP, Herrlich P, Ponta H. Differential expression of CD44 splice variants in intestinal- and diffuse-type human gastric carcinomas and normal gastric mucosa. Cancer Res. 1993 Sep 15;53 (18):4197-203
Heider KH, Hofmann M, Hors E, van den Berg F, Ponta H, Herrlich P, Pals ST. A human homologue of the rat metastasis-associated variant of CD44 is expressed in colorectal carcinomas and adenomatous polyps. J Cell Biol. 1993 Jan;120 (1):227-33
Hofmann M, Rudy W, Zoller M, Tolg C, Ponta H, Herrlich P, Gunthert U. CD44 splice variants confer metastatic behavior in rats: homologous sequences are expressed in human tumor cell lines. Cancer Res. 1991 Oct 1;51 (19):5292-7
Humbria A, Diaz-Gonzalez F, Campanero MR, Arroyo AG, Laffon A, Gonzalez-Amaro R, Sanchez-Madrid F. Expression of L-selectin, CD43, and CD44 in synovial fluid neutrophils from patients with inflammatory joint diseases. Evidence for a soluble form of L-selectin in synovial fluid. Arthritis Rheum. 1994 Mar;37 (3):342-8
Joensuu H, Ristamaki R, Klemi PJ, Jalkanen S. Lymphocyte homing receptor (CD44) expression is associated with poor prognosis in gastrointestinal lymphoma. Br J Cancer. 1993 Aug;68 (2):428-32
Koopman G, Heider KH, Horst E, Adolf GR, van den Berg F, Ponta H, Herrlich P, Pals ST. Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44. J Exp Med. 1993 Apr 1;177 (4):897-904
Kuppner MC, Van Meir E, Gauthier T, Hamou MF, de Tribolet N. Differential expression of the CD44 molecule in human brain tumours. Int J Cancer. 1992 Feb 20;50 (4):572-7
Matsumura Y, Tarin D. Significance of CD44 gene products for cancer diagnosis and disease evaluation. Lancet. 1992 Oct 31;340 (8827):1053-8
Moller P, Eichelmann A, Mechtersheimer G, Koretz K. Expression of beta 1-integrins, H-CAM (CD44) and LECAM-1 in primary gastro-intestinal B-cell lymphomas as compared to the adhesion receptor profile of the gut-associated lymphoid system, tonsil and peripheral lymph node. Int J Cancer. 1991 Dec 2;49 (6):846-55
Omary MB, Trowbridge IS, Letarte M, Kagnoff MF, Isacke CM. Structural heterogeneity of human Pgp-1 and its relationship with p85. Immunogenetics. 1988;27 (6):460-4
Salmi M, Gron-Virta K, Sointu P, Grenman R, Kalimo H, Jalkanen S. Regulated expression of exon v6 containing isoforms of CD44 in man: downregulation during malignant transformation of tumors of squamocellular origin. J Cell Biol. 1993 Jul;122 (2):431-42
Stamenkovic I, Aruffo A, Amiot M, Seed B. The hematopoietic and epithelial forms of CD44 are distinct polypeptides with different adhesion potentials for hyaluronate-bearing cells. EMBO J. 1991 Feb;10 (2):343-8
Underhill C. CD44: the hyaluronan receptor. J Cell Sci. 1992 Oct;103 ( Pt 2):293-8

By Research Topic

Cell adhesion proteins
Cell surface proteins (sCD)
Extracellular matrix
Oncology

By Molecule

CD44

By Technical Data