Type
Recombinant protein
Description
SARS-CoV-2 Spike glycoprotein, Californian Variant B.1.429. Active trimeric form. UniProt acc. no. P0DTC2. Full-length sequence aa 1 –1273. Mutations: S13I, W152C, L452R, D1183Y. Further mutations: furin cleavage site RRAR modified to GSAG, K986P, V987P. C-terminal tag.
Source
HEK293
Purity
98% as determined by SDS-PAGE
Formulation
Liquid in buffer 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001% LMNG
Reconstitution
Defrost at ambient temperature.
Applications
Western blotting, ELISA, COVID-19, ligand binding assays
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store protein at -80°C. Protein remains stable until the expiry date when stored at -80°C. Avoid repeated freezing/thawing cycles.
Note
This product is intended for research use only.