Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate
Sample Requirements
35 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
1–50 ng/ml
Limit of Detection
0.012 ng/ml
Intra-assay (Within-Run)
n = 8; CV = 5.9%
Inter-assay (Run-to-Run)
n = 5; CV = 7.6%
Spiking Recovery
91,00%
Dilution Linearity
96,10%
Crossreactivity
- bovine Non-detectable
- cat Non-detectable
- dog Non-detectable
- goat Non-detectable
- hamster Non-detectable
- horse Yes (recommended dilution 1:6)
- rabbit Non-detectable
- rat Non-detectable
- sheep Non-detectable
- chicken Not tested
- human Yes
- monkey Yes (recommended dilution 1:3)
- mouse Yes (recommended dilution 1:3)
- pig Yes (recommended dilution 1:3)
Note
The kits are CE-IVD certified and intended for professional use.
More about Resistin on BioVendor Scientific Blog
Features
- European Union: for in vitro diagnostic use
- Rest of the world: for research use only!
- The total assay time is less than 4 hours
- The kit measures total resistin in serum and plasma (EDTA, citrate, heparin)
- Assay format is 96 wells
- Quality Controls are human serum based
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Research topic
Diabetology - Other Relevant Products, Energy metabolism and body weight regulation
Summary
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteinerich
secreted proteins which is termed the RELM family, and is also described as ADSF
(Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone). Human
resistin contains 108 amino acids as a prepeptide, and its hydrophobic signal peptide is
cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting
of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Much of the early investigations about the resistin molecule are based on the mouse model.
Resistin, produced and secreted primarily by adipocytes in mice, acts on skeletal muscle
myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin.
Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate
glucose uptake. Other studies have shown that mouse resistin increases during the
differentiation of adipocytes, but it also seems to inhibit adipogenesis.
Compared to the mouse model, human adipogenic differentiation is likely to be associated with
a down regulation of resistin gene expression. On the other hand, resistin was found to be
expressed at high levels in human monocytes, macrophages and bone marrow.
Recent investigations have shown that human resistin is correlated with metabolic syndrome
and obesity-related disorders. Malo et al. have reported that resistin levels are positively
associated with waist circumference, tumor necrosis factor-a, and insulin resistance assessed
by the homeostasis model, and inversely correlated with total cholesterol, HDL cholesterol, and
LDL cholesterol. Moreover, Sadhasiv et al. found a positive correlation of SAT (subcutaneous
adipose tissue) resistin mRNA expression with serum resistin, BMI and insulin resistance
(HOMA index).
Based on the above reports human resistin might be an important marker that acts as the link
between obesity and insulin resistance. Resistin can play a role also in inflammation processes
and in atherosclerosis.
Clinical use and areas of investigation: Energy metabolism and body weight regulation, Metabolic syndrome, Inflammation, Atherosclerosis
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
Product Brochure
CE IVD Assays
Other Documents
Declaration of Conformity
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