Type
Competitive ELISA, Immobilized antibody
Description
The Immunodiagnostic Systems Limited Rat/Mouse PINP EIA kit is a competitive enzyme immunoassay (EIA) for the quantitative determination of N-terminal propeptide of type I procollagen (PINP) in rat or mouse serum, plasma (Heparin or EDTA), or cell culture media samples. PINP is a particularly useful marker for monitoring the efficacy of osteoporosis therapy with anabolic agents, but it is also one of the best bone turnover markers for monitoring the efficacy of antiresorptive therapy.
Scientific Description:
The Rat/Mouse PINP EIA assay is a specific method to determine PINP released during rat and mouse bone collagen synthesis, and it has no cross-reactivity for human PINP. The Rat/Mouse PINP EIA assay can be used for determining the bone formation rate from rat and mouse serum samples, and from the culture medium in rat and mouse osteoblast cultures. Previous studies have demonstrated that PINP secretion is increased after stimulation of mouse osteoblasts by BMP-4 and oestrogen in vitro. PINP values are also elevated after treatment with PTH in both young ovariectomised rats and old intact rats.
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
5 μL, predilution 1:10
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
0.7 – 75 ng/mL