Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.246 - 60 ng/ml
Limit of Detection
200 pg/ml
Intra-assay (Within-Run)
CV <10%
Inter-assay (Run-to-Run)
CV < 12%
Spiking Recovery
serum 98.54%, plasma 121.5%, cell culture media 112.2%
Dilution Linearity
serum 86.5%, plasma 75.42%, cell culture media 83.48%
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha
Features
- RUO
- calibration range 0.246 - 60 ng/ml
- limit of detection 200 pg/ml
- intra-assay CV <10%
- inter-assay CV < 12%
Research topic
Immune Response, Infection and Inflammation, Sepsis, Animal studies
Summary
The BioVendor Rat CRP (C Reactive Protein) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CRP in serum, plasma, cell culture supernatants. This assay employs an antibody specific for Rat CRP coated on a 96-well plate. Standards and samples are pipetted into the wells and CRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiRat CRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Instructions for Use (RUO)
Instructions for Use (RUO)
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