Type
ELISA
Description
The QuickZyme human MMP-9 activity assay enables you to specifically measure in biological samples both active human MMP-9, as well as (pro)MMP-9 which is activated on the plate by APMA. It can be used for the measurement of MMP-9 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine.
Applications
Serum, Urine, Plasma, Tissue homogenates, Cell culture conditioned medium
Sample Requirements
10 - 100 µl
Shipping
On dry ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Unopened kit:
Store at -20°C, except for the standard, this vial should be stored at -70°C. Do not use kit, or individual kit components past kit expiration date.
Opened kit / reconstituted reagents:
Please refer to kit manual.
Calibration Range
0 - 16 ng/ml
Limit of Detection
0.1 ng/ml (1 hr incubation)
0.005 ng/ml (4 hr incubation)
Features
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Measures endogenous active MMP-9 ( naturally occurring ) or total active MMP-9 ( following activation with APMA ).
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Samples: conditioned cell culture medium, serum, plasma, urine, synovial fluid and tissue homogenates.
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Quantitative.
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Range: 0.5 to 16 ng/ml (1 h incubation with detection reagent), 0.01-1 ng/ml (4 h incubation with detection reagent).
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Sensitivity: 0.005 ng/ml.
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Ease-of-use: Equivalent to ELISA.
Research topic
Bone and cartilage metabolism, Cardiovascular disease, Extracellular matrix, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Others, Pulmonary diseases
Summary
Matrix metalloproteinases (MMPs) are a family of enzymes that function in the remodeling of
extracellular matrix proteins. They are essential for various normal physiological processes
such as embryonic development, morphogenesis, reproduction tissue resorption and tissue
remodeling. They also play a role in a number of pathological processes such as
inflammation, arthritis, cardiovascular diseases, fibrosis and cancer.
Regulation of MMPs is carried out at various levels. Expression of latent MMPs is regulated
at the level of transcription, whereas the proteolytic activity is controlled by specific activation
of proMMPs, and by MMP-specific inhibitors, the tissue inhibitors of metalloproteinases
(TIMPs) or general circulatory inhibitors, such as α2macroglobulin.
The MMPs can be grouped according to their domain structure into
collagenases, gelatinases, stromelysins, membrane type MMPs and matrilysins.
MMP-9 (also known as type neutrophil gelatinase, IV collagenase, Gelatinase B;
EC 3.4.24.35) has a broad range of substrate specificity for denatured collagens (gelatins),
native collagens (types IV, V and XI), as well as elastin.
Human MMP-9 has a Mw of 92 kDa (pro-form) and 82 kDa (active form). The activity is
dependent on Zn2+ and Ca2+. MMP-9 is secreted as proMMP-9, and can be activated in vitro
by organo mercurial compounds such as p-aminophenyl mercuric acetate (APMA).
MMP-9 is produced by a variety of cell types including monocytes, macrophages, fibroblasts,
neutrophils, osteoclasts, chondrocytes, keratinocytes, endothelial and epithelial cells.
The QuickZyme human MMP-9 activity assay enables you to specifically measure in
biological samples both active human MMP-9, as well as (pro)MMP-9 which is activated on
the plate by APMA. It can be used for the measurement of MMP-9 activity in various
biological samples, such as conditioned culture media, tissue homogenates, serum, plasma
and urine.
