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PMN Elastase Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Elastase-2, Leukocyte elastase, Neutrophil elastase
  • Species:Human
Cat. No. Size Price


RM191021100 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Description

The test kit is a solid phase enzyme-linked immunosorbent assay (ELISA) in the microplate format, designed for the quantitative measurement of the complex of human PMN Elastase and α1-proteinase inhibitor (α1-PI) in blood plasma and seminal plasma. The microplate is coated with a polyclonal antibody directed against human PMN Elastase (antigen).

Applications

Plasma-EDTA, Plasma-Citrate, Seminal plasma

Sample Requirements

10 µl/well

Shipping

On blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Storage conditions for aliquots of Standards, Quality Controls and samples are – 20°C. Shelf life of components is 30 days after opening or until the expiration date.

Calibration Curve

Calibration Range

0.16 –10 ng/ml

Limit of Detection

0.03 ng/ml

Summary

Features

  • ** Ror research use only!**
  • The total assay time is less than 3 hours.
  • The kit measures PMN elastase in plasma (EDTA and citrate) and seminal plasma.
  • Assay format is 96 wells.
  • Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Immune Response, Infection and Inflammation

Summary

The BioVendor PMN Elastase ELISA is an enzyme immunoassay for the quantitative determination of the complex of PMN Elastase and the α1-proteinase inhibitor (α1-PI) in human EDTA or citrated plasma and seminal plasma. The assay is intended for research use only. Manual processing is validated. The usage of laboratory automates is the user’s sole responsibility. The kit is intended for single use only.

Product References (1)

References

  • Alexandrovski M, Suciu S, Alexandrovski J. Joint Measurements of Leukocyte Elastase and Myeloperoxidase Promote Identification of the State of Neutrophils in Diabetic Patients. Biores Open Access. 2020 Aug 20;9(1):190-197. doi: 10.1089/biores.2020.0012. eCollection 2020. PubMed PMID: 32908806. PubMed CentralPMCID: PMC7473041. See more on PubMed
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