Type
Sandwich ELISA, HRP-labelled antibody
Description
The RIS008R Human Pepsinogen II ELISA Enzyme Linked ImmunoSorbent Assay (ELISA)
for the measurement of human pepsinogen II levels in serum. It is intended for research use
only.
This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative
determination of human pepsinogen II levels in serum. Determination of human serum
pepsinogen II level would allow calculation of the ratio of pepsinogen I/II, which was reported to
be a useful tool in the aid of diagnosing the functional states of acid secreting gastric mucosa.
Applications
Serum
Sample Requirements
50 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
6.3–100 ng/ml
Limit of Detection
0.1 ng/ml
Specificity
This assay measures human pepsinogen II without any cross-reaction to human pepsinogen I.
Features
- RUO
- calibration range 6.3-100 ng/ml
- limit of detection 0.1 ng/ml
- for the measurement of human pepsinogen II levels in serum
- controls
Research topic
Others
Summary
Pepsinogen consists of a single polypeptide chain of 375 amino acids with an average
molecular weight of 42 kDa. Pepsinogen I is synthesized at gastric chief cells and mucous
neck cells, while pepsinogen II is produced not only by gastric chief cells and mucous neck
cells, but also by clear mucous cells of antrum and Brunner’s glands in the proximal
duodenum, etc. The clinical applications of measuring pepsinogen I and pepsinogen II are a
useful aid in diagnosing severe atrophic gastritis and stomach cancer. It was suggested that
the measurement of serum pepsinogens served as a “serological biopsy” for predicting the
presence of atrophic gastritis or superficial gastritis.
Atrophic Gastritis: It was found that serum pepsinogen I level falling to less than 20 ng/ml was
highly specific for severe atrophic gastritis. It is also observed that serum pepsinogen I levels
fell with increasing severity of mucosal damage in atrophic gastritis. The diagnostic sensitivity
and specificity of serum pepsinogen I level for advanced atrophic corpus gastritis are about
92% and 90% respectively. On the other hand, the decrease in serum pepsinogen I levels in
patients with pernicious anemia and atrophic gastritis was found to be associated with normal
or raised pepsinogen II levels. Therefore, a pepsinogen I/pepsinogen II ratio is significantly
lower than those with superficial gastritis or normal remnant mucosa.
Stomach Cancer: Low serum pepsinogen I levels as well as low ratio of pepsinogen I/II were
found in patients with gastric cancer, with a threefold higher incidence. Other studies have
concluded that low serum pepsinogen I levels may identify persons at increased risk for
intestinal types of stomach cancer.
Duodenal Ulcer: A low serum pepsinogen I level can exclude a diagnosis of duodenal ulcer.
Although a high pepsinogen I level has less clinical use for establishing the diagnosis of a
duodenal ulcer, the combination of hypergastrinemia and a highly elevated serum pepsinogen I
strongly suggests the possibility of the Zollinger-Ellison syndrome.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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