Type
Polyclonal Antibody
Source of Antigen
E. coli
Hosts
Sheep
Isotype
IgG
Preparation
The antibody was raised in sheep by immunization with the recombinant Human NT-proBNP.
Amino Acid Sequence
Recombinant Human NT-proBNP, total 83 AA. MW: 9.4 kDa (calculated). UniProtKB acc. no. P16860 (His27–Arg102). N-terminal His-tag (7 extra AA).
MKHHHHHHPLSPGSASDLETSGLQEQRNHLQGKLSELQVEQTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPR
Species Reactivity
Human. Not yet tested in other species.
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Human NT-proBNP.
Antibody Content
0.1 mg (determined by BCA method, BSA was used as a standard)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2.
Reconstitution
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Quality Control Test
Indirect ELISA – to determine titer of the antibody SDS PAGE – to determine purity of the antibody BCA - to determine quantity of the antibody
Note
This product is for research use only.