Type
Recombinant protein
Description
Total 668 AA. MW 76.3 kDa (calculated). UniProtKB acc. No. P20591 (Met1-Gly662). C-terminal His-tag (6 extra AA). Protein identity confirmed by MS.
Amino Acid Sequence
MVVSEVDIAKADPAAASHPLLLNGDATVAQKNPGSVAENNLCSQYEEKVRPCIDLIDSLRALGVEQDLALPAIAVIGDQSSGKSSVLEALSGVALPRGSGIVTRCPLVLKLKKLVNEDKWRGKVSYQDYEIEISDASEVEKEINKAQNAIAGEGMGISHELITLEISSRDVPDLTLIDLPGITRVAVGNQPADIGYKIKTLIKKYIQRQETISLVVVPSNVDIATTEALSMAQEVDPEGDRTIGILTKPDLVDKGTEDKVVDVVRNLVFHLKKGYMIVKCRGQQEIQDQLSLSEALQREKIFFENHPYFRDLLEEGKATVPCLAEKLTSELITHICKSLPLLENQIKETHQRITEELQKYGVDIPEDENEKMFFLIDKVNAFNQDITALMQGEETVGEEDIRLFTRLRHEFHKWSTIIENNFQEGHKILSRKIQKFENQYRGRELPGFVNYRTFETIVKQQIKALEEPAVDMLHTVTDMVRLAFTDVSIKNFEEFFNLHRTAKSKIEDIRAEQEREGEKLIRLHFQMEQIVYCQDQVYRGALQKVREKELEEEKKKKSWDFGAFQSSSATDSSMEEIFQHLMAYHQEASKRISSHIPLIIQFFMLQTYGQQLQKAMLQLLQDKDTYSWLLKERSDTSDKRKFLKERLARLTQARRRLAQFPGHHHHHH
Source
E. coli
Purity
˃ 90 % by SDS-PAGE
SDS-PAGE Gel
12 % SDS-PAGE separation of Human MxA (E.coli):
- M.W. marker – 14, 21, 31, 45, 66, 97 kDa
- Reduced and boiled sample, 2.5 μg/lane
- Non-reduced and non-boiled sample, 2.5 μg/lane
Endotoxin
< 1.0 EU/µg
Formulation
Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in 20 mM Tris buffer, 50 mM NaCl, 5% (w/v) trehalose, pH 7.5.
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely at 37°C.
Applications
Western blotting, ELISA
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the lyophilized protein at -80 °C. Lyophilized protein remains stable until the expiry date when stored at -80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80 °C for long term storage. Reconstituted protein can be stored at 4 °C for three days.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Endotoxin level determination.
Note
This product is intended for research use only.
Research topic
Immune Response, Infection and Inflammation, Sepsis, COVID-19
Summary
Human MxA protein (Myxovirus resistance protein 1), encoded by the MX1 gene, is a 76-kDa protein composed of 662 amino acid residues and is a member of the dynamin superfamily of large GTPases. The MxA protein plays a crucial role in antiviral defense within cells, providing protection against a broad range of viruses, including influenza, parainfluenza, measles, coxsackie, hepatitis B, and Thogoto viruses.
The viruses are inhibited by MxA protein at an early stage in their life cycle, soon after host cell entry and before genome amplification. The human MxA protein is accumulated in the cytoplasm and endoplasmic reticulum. The membrane compartment of endoplasmatic reticulum seems to provide an interaction platform that facilitates viral target recognition. MxA appears to detect viral infection by sensing and trapping nucleocapsid structures, and becoming the viral components unavailable for the generation of new virus particles.The expression of viral MxA protein is induced exclusively and, in a dose-dependent manner, by IFN-alpha and IFN-beta, but not by IFN-gamma, IL-1, TNF-alpha or other cytokines.
MxA protein may offer advantages as a laboratory marker because of its very low basal concentration, increasing within 1-2 hours of infection and long half-life being approximately 2-3 days. In mononuclear cells stimulated with high doses of leukocyte IFN-alpha, MxA mRNA levels increased tenfold within 4 hours, and elevated MxA protein levels persists over the next 48 hours. MxA protein with its low basal concentration and long half-life, offers advantages as a marker for viral infection. Clinical studies have reported on MxA protein in peripheral blood mononuclear cells as a marker distinguishing viral from bacterial disease, and as a reliable marker for type I IFN bioavailability during IFN treatment in patients with multiple sclerosis (MS) according to the recommendation from EMA (European Medicine Agency).
Main Clinical Use of MxA
- Differentiating viral from bacterial infections
- Detection and assessment of active phase of Viral Infections
- Immune response assessment
- Autoimmune and inflammatory disease activity marker
- Therapeutic monitoring in interferon therapy
