Type
Recombinant protein
Description
Monocyte Chemotactic Protein-1Human Recombinant also known as Monocyte Chemotactic and Activating Factor (MCAF) produced in E.coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8607 Dalton. The MCP-1 is purified by proprietary chromatographic techniques.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Ile.
Source
E. coli
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE
Biological Activity
The specific activity as determined by the ability of MCP-1 to chemoattract human Monocytes using a concentration of 5–20 ng/ml.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL2 should be stored at 4°C between 2–7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance
Sterile filtered white lyophilized (freeze-dried) powder.