Type
Competitive ELISA, Immobilized antigen
Description
The Irisin ELISA Kit is to be used for the in vitro quantitative determination of irisin in serum, plasma and cell culture supernatant of human origin. It should also work for the in vitro quantitative determination of irisin in mouse, rat and monkey biological samples. This ELISA Kit is for research use only.
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
50 ul/wells
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.001–5 μg/ml
Limit of Detection
1 ng/ml
Intra-assay (Within-Run)
CV = 6,9%
Inter-assay (Run-to-Run)
CV = 9.1%
Spiking Recovery
109%
Dilution Linearity
90-107%
Specificity
This ELISA is specific for the measurement of natural and recombinant irisin in human samples. It should also work in mouse, rat and monkey biological samples. It does not cross-react with FNDC4, human adiponectin, human Nampt, human RBP4, human clusterin, human leptin, human vaspin, human GPX3, human resistin, human ACE2, human lipocalin-2, human ANGPTL3, human ANGPTL6, human DNER, human DLK1, human calreticulin, human IL-33, mouse Nampt, mouse clusterin, mouse vaspin, mouse resistin.
Features
- RUO
- calibration range 0.001-5 ug/ml
- limit of detection 1 ng/ml
Research topic
Energy metabolism and body weight regulation, Animal studies
Summary
Fibronectin type III domain containing (FNDC) family consists of the multiple protein species but has a typified fibronectin type III domain (1-2). Among them FNDC5 also called irisin, hereinafter named irisin, has been identified a myokine which is capable of inducing browning of white adipose tissue leading to brown adipocytes and beige fat cells by stimulating UCP1 expression, via the PGC-1α transcriptional co-activator (3). Irisin is a single-pass type I membrane protein whose secretion could be by a putative signal peptide. Since irisin has been discovered as a soluble protein from the culture supernatant of the myocytes from the muscle-specific PGC-1α transgenic mice the predicted extracellular domain should be cleaved followed by being liberated from the cell surface membrane. Indeed, this was the case by having shown that the presence of the irisin exists in the culture supernatants and human/mouse sera as a 22 kDa protein (3). Irisin is able to induce UCP1 as well as a number of genes manifested in brown adipose tissues. In particular, the level of irisin was increased by muscular exercise in mouse and human, suggesting that irisin plays an important role in obesity and glucose homeostasis.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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