Type
Recombinant protein
Description
IP-10 Mouse Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8701 Dalton. The IP-10 is purified by proprietary chromatographic techniques.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ile-Pro-Leu-Ala-Arg.
Source
E. coli
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity
The Biological activity is calculated by its ability to chemoattract IL-2 activated T cells at 0.1–10 ng/ml.
Formulation
The CXCL10 protein was lyophilized with no additives.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Lyophilized IP-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL10 should be stored at 4°C between 2–7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Research topic
Cytokines and chemokines and related molecules, Immune Response, Infection and Inflammation, Animal studies
Summary
IP-10 (Interferon-gamma inducible Protein 10kDa) also known as CXCL10, is secreted by several cell types in response to IFNg and LPS. These cell types include monocytes, endothelial cells and fibroblasts.The gene for IP-10 is located on chromosome 4 in a cluster among several other cytokines and encodes a 98 amino acid precursor protein. IP-10 has been attributed to several roles, such as chemoattraction for monocytes and T cells (but not for neutrophils), inhibition of bone marrow colony formation and angiogenesis, promotion of T cells adhesion molecule expression. IP-10 shares a common receptor, CXCR3, with the chemokine MIG, but has been shown to play a distinct role in host defense in infections. IP-10 expression has been associated with HIV infection, is involved in inflammatory skin disease (6) and other allergic diseases; it appears in inflammation of the nervous system and in Alzheimer’s disease (astrocytes expressing IP-10 are commonly associated with senile plaques).
Product Datasheet (RUO)
Datasheet PDF (RUO)
Datasheet PDF (RUO)
Safety Information (RUO)
MSDS (RUO)
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