Interleukin-6 Rat ELISA

Cat. No.	Size	Price
RAF072R	96 wells (1 kit)	$694,84

Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Cell culture supernatant

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

31.0–2000 pg/ml

Limit of Detection

12.0 pg/ml

Intra-assay (Within-Run)

CV = 5.0%

Inter-assay (Run-to-Run)

CV = 10.0%

Dilution Linearity

97,00%

Summary

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Sepsis, Animal studies, COVID-19

Summary

Interleukin-6 is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis, and it may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide or viral infections.

Product References (3)

References

Trinchese G, Cavaliere G, Penna E, De Filippo C, Cimmino F, Catapano A, Musco N, Tudisco R, Lombardi P, Infascelli F, Messina G, Muredda L, Banni S, Monda M, Crispino M, Mollica MP. Milk From Cow Fed With High Forage/Concentrate Ratio Diet: Beneficial Effect on Rat Skeletal Muscle Inflammatory State and Oxidative Stress Through Modulation of Mitochondrial Functions and AMPK Activity. Front Physiol. 2019 Jan 17;9:1969. doi: 10.3389/fphys.2018.01969. eCollection 2018. PubMed PMID: 30705640. PubMed CentralPMCID: PMC6344429. See more on PubMed
Cavaliere G, Viggiano E, Trinchese G, De Filippo C, Messina A, Monda V, Valenzano A, Cincione RI, Zammit C, Cimmino F, Catapano A, Sessa F, Messina G, Monda M, Crispino M, Mollica MP. Long Feeding High-Fat Diet Induces Hypothalamic Oxidative Stress and Inflammation, and Prolonged Hypothalamic AMPK Activation in Rat Animal Model. Front Physiol. 2018 Jul 6;9:818. doi: 10.3389/fphys.2018.00818. eCollection 2018. PubMed PMID: 30034345. PubMed CentralPMCID: PMC6043859. See more on PubMed
Peric Kacarevic Z, Snajder D, Maric A, Bijelic N, Cvijanovic O, Domitrovic R, Radic R. High-fat diet induced changes in lumbar vertebra of the male rat offsprings. Acta Histochem. 2016 Sep;118(7):711-721. doi: 10.1016/j.acthis.2016.08.002. Epub 2016 Aug 28. PubMed PMID: 27577321. See more on PubMed

Summary References (7)

References to Interleukin-6

Cayphas S, Van Damme J, Vink A, Simpson RJ, Billiau A, Van Snick J. Identification of an interleukin HP1-like plasmacytoma growth factor produced by L cells in response to viral infection. J Immunol. 1987 Nov 1;139 (9):2965-9
Hirano T, Akira S, Taga T, Kishimoto T. Biological and clinical aspects of interleukin 6. Immunol Today. 1990 Dec;11 (12):443-9
Hirano T, Kishimoto T. Interleukin-6.Handbook of experimental pharmacology, Peptide Growth Factors and Their Receptors. Springer-Verlag. 1990;:633-665
Hirano T, Taga T, Nakano N, Yasukawa K, Kashiwamura S, Shimizu K, Nakajima K, Pyun KH, Kishimoto T. Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). Proc Natl Acad Sci U S A. 1985 Aug;82 (16):5490-4
Koj A. The Role of Interleukin-6 as the Hepatocyte Stimulating Factor in the Network of Inflammatory Cytokines. Annals of the New York Academy. (1989);:557: 1–8
Nordan RP, Potter M. A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. Science. 1986 Aug 1;233 (4763):566-9
Ray A, Tatter SB, Santhanam U, Helfgott DC, May LT, Sehgal PB. Regulation of expression of interleukin-6. Molecular and clinical studies. Ann N Y Acad Sci. 1989;557:353-61; discussion 361-2

Type

Applications

Sample Requirements

Shipping

Storage/Expiration

Calibration Curve

Calibration Range

Limit of Detection

Intra-assay (Within-Run)

Inter-assay (Run-to-Run)

Dilution Linearity

Research topic

Summary

References

References to Interleukin-6

Instructions for Use (RUO)

Safety Information (RUO)

Find documents for the lot

Interleukin-6 Rat ELISA

Type

Applications

Sample Requirements

Shipping

Storage/Expiration

Calibration Curve

Calibration Range

Limit of Detection

Intra-assay (Within-Run)

Inter-assay (Run-to-Run)

Dilution Linearity

Research topic

Summary

References

References to Interleukin-6

By Research Topic

By Molecule

By Technical Data

Instructions for Use (RUO)

Safety Information (RUO)

Find documents for the lot