Type
Sandwich ELISA, Biotin-labelled antibody
Description
The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5. The human IL-5 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Applications
Serum, Cell culture supernatant
Sample Requirements
50 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
7.8–500 pg/ml
Limit of Detection
1.5 pg/ml
Intra-assay (Within-Run)
CV = 6.6%
Inter-assay (Run-to-Run)
CV = 6.8%
Spiking Recovery
74%
Dilution Linearity
110%
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-5 positive serum.
There was no crossreactivity detected.
Features
- RUO
- calibration range: 7.8-500 pg/ml
- limit of detection 1.5 pg/ml
- intra-assay CV = 6.6%
- inter-assay CV = 6.8%
Research topic
Cytokines and chemokines and related molecules
Summary
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has
been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein with a native molecular weight of 45 to 60 kDa, with multiple subunits of 24 to 28 kDa under reducing conditions.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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