Type
Sandwich ELISA, Biotin-labelled antibody
Description
The mouse IL-1ß ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-1ß. The mouse IL-1ß ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Applications
Serum, Cell culture supernatant
Sample Requirements
50 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
7.8–500 pg/ml
Limit of Detection
1.2 pg/ml
Intra-assay (Within-Run)
CV = 4.7%
Inter-assay (Run-to-Run)
CV = 5.7%
Spiking Recovery
77,00%
Dilution Linearity
109,00%
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-1ß positive serum.
There was no crossreactivity detected.
Features
- RUO
- calibration range 7.8 - 500 pg/ml
- limit of detection 1.2 pg/ml
- intra-assay CV = 4.7%
- inter-assay CV = 5.7%
Research topic
Cardiovascular disease, Coronary artery disease, Cytokines and chemokines and related molecules, Diabetology - Other Relevant Products, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Transplantation, Animal studies, COVID-19
Summary
Interleukin-1 (IL-1), originally described in 1972 as lymphocyte activating factor (LAF) for its effects on thymocytes, is a polypeptide cytokine with two molecular forms. Both forms appear to mediate identical ranges of biological activity which include synthesis of the acute phase proteins by hepatocytes, chemotaxis of polymorphonucleocytes, and release of polymorphonucleocytes from blood and bone marrow. These effects coined the acronym leukocyte endogenous mediator (LEM). Early researchers also called IL-1ß endogenous pyrogen, and it has been shown to induce fever and is thought to contribute to wasting of muscles (PIF, proteolysis inducing factor). Other activities associated with IL-1 are the induction of Prostaglandin E2 by synovial cells and release of collagenase with resulting destruction of cartilage and bone resorption (catabolin, osteoclast activation factor). In addition, IL-1, has multiple immunological functions including enhancement of IL-2 production by T cells and activation of B-cells (BAF) and thymocytes. A true pleiotrope, IL-1 may have tumoricidal activity via its release of IL-2 and interferon gamma and be indirectly antiviral by stimulating fibroblasts to release interferon beta. In its role as mediator of sepsis, IL-1 has most recently been described as enhancing the growth of virulent E. coli.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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