Cat # changed from 633-02899 to 639-02891
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma
Sample Requirements
5 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
1–100 ng/ml
Limit of Detection
50 pg/ml
Research topic
Asthma and allergic rhinitis, Immunology, Animal studies
Summary
IgE (Immunoglobulin E) is a glycoprotein with a molecular size of 190 kDa composed of 2 light chains and 2 heave chains (H ). In electrophoresis, it moves to γ1 region. Its biological half life is about 3 days, and its blood level in normal human subject is very low, about 300 ng/ml. The blood level of IgE increases markedly in parasite infection and in hay fever. IgE that is responsible for allergy has been called “reagin”. Sensitization by an allergen increases reagin IgE which binds to FcεR1 receptor in basophilic leucocytes and mast cells at Fc region and sensitizes those cells. If the allergen binds the sensitized cells, those cells will be degranulated and release histamin, serotonin, protease, heparin, chemotactic factor, prostaglandins, leucotriens, and so on, causing bronchoconstriction, mucous edema, and hypersecretion, and leads to type I allergic reactions like bronchial asthma, hives, allergic rhinitis, anaphylaxis, and so on. This is the assay kit for total mouse IgE. Shibayagi is also providing assay kits for allergen(OVA)-specific mouse IgE.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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