Type
Sandwich ELISA, Biotin-labelled antibody
Description
The assay is based on polyclonal antibodies and recombinant IGFBP-6,
expressed in eukaryotic cells. The ELISA for IGFBP-6, E112 is a so-called
Sandwich-Assay.
Applications
Serum, Plasma, Body fluids
Sample Requirements
10 μl
Shipping
Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
0.1 – 10 ng/ml
Limit of Detection
0.026 ng/ml
Intra-assay (Within-Run)
<10 %
Inter-assay (Run-to-Run)
<10 %
Features
For Research Use Only
incubation period 3 hours
buffer : RTU and 20fold concentrate
2 control sera, freeze-dried
Intra- / Interassay Variance <10 %
Research topic
Animal studies
Summary
The Insulin-like Growth Factor 6 (IGFBP-6) is part of the IGF-System which consists of
several binding proteins (1-6) and IGF-I and –II. The Insulin-like Growth Factors are involved
in the control of human growth and regulated themselves by their binding proteins. The
IGFBPs show high amino acid sequence homology, they have conserved N- and C-terminal
domains which are involved in IGF-binding. In contrast the central linker region is highly
variable. This region is not directly involved in IGF-binding but influences stability and
localisation of the IGFBP / IGF complex and can be modified by glycosylation and
phosphorylation.
Find documents for the lot
Example Instructions for Use (RUO)
Example Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
MSDS (RUO)
