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Manufactured by BioVendor

Human Tie-2 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Angiopoietin-1 receptor, Tyrosine-protein kinase receptor TEK
  • Species:Human
Cat. No. Size Price


New REH011R 96 wells (1 kit) $518,5
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma, Cell culture supernatant

Shipping

On blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

20.58 - 15 000 pg/ml

Limit of Detection

20 pg/ml

Intra-assay (Within-Run)

CV < 10%

Inter-assay (Run-to-Run)

CV < 12%

Spiking Recovery

serum 110.9%, plasma 104,2%, cell culture media 76.59%

Dilution Linearity

serum 79.43%, plasma 80.43%, cell culture media 77.65%

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFNgamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Summary

Features

  • RUO
  • calibration range 20.58 - 15 000 pg/ml
  • limit of detection 20 pg/ml
  • intra-assay CV <10%
  • inter-assay CV < 12%

Summary

The BioVendor Human Tie-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Tie-2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Tie-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Tie-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Tie-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tie-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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