Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
40.96 - 10 000 pg/ml
Limit of Detection
40 pg/ml
Intra-assay (Within-Run)
CV <10%
Inter-assay (Run-to-Run)
CV <12%
Spiking Recovery
serum 123.3%, plasma 125.7%, cell culture media 96.04%
Dilution Linearity
serum 80.32%, plasma 77%, cell culture media 104.2%
Specificity
This ELISA antibody pair detects human SOST. Other species not determined yet.
Features
- RUO
- calibration range 40.96 - 10 000 pg/ml
- limit of detection 40 pg/ml
- intra-assay CV <10%
- inter-assay CV < 12%
Research topic
Bone and cartilage metabolism
Summary
The BioVendor Human SOST (also known as sclerostin) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SOST in serum serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human SOST coated on a 96-well plate. Standards and samples are pipetted into the wells and SOST present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human SOST antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SOST bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Instructions for Use (RUO)
Instructions for Use (RUO)
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