Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.066- 16 ng/ml
Limit of Detection
0.06 ng/ml
Intra-assay (Within-Run)
CV <10%
Inter-assay (Run-to-Run)
CV <12%
Spiking Recovery
serum 113.2%, plasma 106.3%, cell culture media 129.9%
Dilution Linearity
serum 94.39%, plasma 96.07%, cell culture media 106.4%
Specificity
This ELISA antibody pair detects human S100A6. Other species not determined.
Features
- RUO
- calibration range 0.066-16 ng/ml
- limit of detection 0.06 ng/ml
- intra-assay CV <10%
- inter-assay CV <12%
Research topic
Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Others
Summary
The BioVendor Human S100A6 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human S100A6 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human S100A6 coated on a 96well plate. Standards and samples are pipetted into the wells and S100A6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human S100A6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of S100A6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Instructions for Use (RUO)
Instructions for Use (RUO)
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