| Cat. No. | Size |
Price |
|
|---|---|---|---|
| New REH012R | 96 wells (1 kit) | $528 |
Sandwich ELISA, Biotin-labelled antibody
Cell lysate
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
The BioVendor Phosphotyrosine TIE-2 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated TIE-2 in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis. This sandwich-based ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human phospho-TIE-2. An anti-TIE-2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated TIE-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-TIE-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.