Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
6 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
125 - 4 000 pg/ml
Limit of Detection
31 pg/ml
Intra-assay (Within-Run)
n = 8
CV = 3.5%
Inter-assay (Run-to-Run)
n = 8
CV = 4.1%
Spiking Recovery
87.0%
Dilution Linearity
104.2%
Features
It is intended for research use only
The total assay time is less than 5 hours
The kit measures total osteopontin
Assay format is 96 wells
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Bone and cartilage metabolism, Cardiovascular disease, Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Renal disease
Summary
Osteopontin (OPN), a secreted multifunctional phosphoprotein, is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of cell matrix proteins and participates in many biological activities. OPN has been demonstrated to be closely related to the occurrence and development of many bone-related diseases, such as osteoporosis, rheumatoid arthritis, and osteosarcoma. Besides that, it functions as a proinflammatory cytokine and promotes cell-mediated immune responses, and also has protective functions such as biomineralization and wound healing, and is also a strong predictor of adverse outcomes in patients with CVDs. Thus, OPN is not only a risk factor but also a potential therapeutic target for CVDs.
Instructions for Use (RUO)
Instructions for Use (RUO)
Find documents for the lot