Human Obestatin ELISA

Cat. No.	Size	Price
RA19025R	96 wells (1 kit)	$729

Technical Data

Type

Sandwich ELISA, AChE-labelled antibody

Applications

Plasma-EDTA

Sample Requirements

12.5 µl/well

Shipping

On ice. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at -20°C. Under these conditions, the kit is stable until expiration date (see label on the box).

Calibration Range

31.3 - 4000 pg/ml

Limit of Detection

62.5 pg/ml

Intra-assay (Within-Run)

n = 20
CV = 3.7%

Inter-assay (Run-to-Run)

n = 5
CV = 11.9%

Spiking Recovery

90.9%

Dilution Linearity

108.0%

Summary

Features

It is intended for research use only . The total assay time is about 24 hours . The kit measures obestatin in EDTA plasma (K3EDTA)Assay format is 96 wells . Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Energy metabolism and body weight regulation

Summary

Obestatin was identified as an anorexigenic peptide with an action on the food intake. The first studies have shown that the obestatin reduced food intake and body weight. It has also been considered to be an antidiabetic peptide by positively influencing glucose and lipid metabolism. Obestatin reduces the apoptosis and promote the proliferation of B-cells and human pancreatic islets. Due to heterogeneity of these sources, obestatin has many different functions. Indeed obestatin could have a function in the regulation of blood pressure and its plasmatic concentration increases in case of hypertensive patients.

Summary References (7)

References to Obestatin

Dun SL, Brailoiu GC, Brailoiu E, Yang J, Chang JK, Dun NJ. Distribution and biological activity of obestatin in the rat. J Endocrinol. 2006 Nov;191 (2):481-9
Green BD, Irwin N, Flatt PR. Direct and indirect effects of obestatin peptides on food intake and the regulation of glucose homeostasis and insulin secretion in mice. Peptides. 2007 May;28 (5):981-7
Nagaraj S, Peddha MS, Manjappara UV. Fragments of obestatin as modulators of feed intake, circulating lipids, and stored fat. Biochem Biophys Res Commun. 2008 Feb 15;366 (3):731-7
Ren AJ, Guo ZF, Wang YK, Wang LG, Wang WZ, Lin L, Zheng X, Yuan WJ. Inhibitory effect of obestatin on glucose-induced insulin secretion in rats. Biochem Biophys Res Commun. 2008 May 9;369 (3):969-72
Samson WK, White MM, Price C, Ferguson AV. Obestatin acts in brain to inhibit thirst. Am J Physiol Regul Integr Comp. 2007 Jan;292 (1):R637-43
Scrima M, Campiglia P, Esposito C, Gomez-Monterrey I, Novellino E, D'Ursi AM. Obestatin conformational features: a strategy to unveil obestatin's biological role?. Biochem Biophys Res Commun. 2007 Nov 23;363 (3):500-5
Zhang JV, Ren PG, Avsian-Kretchmer O, Luo CW, Rauch R, Klein C, Hsueh AJ. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science. 2005 Nov 11;310 (5750):996-9

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Instructions for Use (RUO)

Safety Information (RUO)

MSDS (RUO)

Product Brochure

Ghrelin and Obestatin: Twin proteins encoded by the one gene