Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
5 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.625 - 20 ng/ml
Limit of Detection
0.16 ng/ml
Intra-assay (Within-Run)
n = 8
CV = 2.5%
Inter-assay (Run-to-Run)
n = 8
CV = 7%
Spiking Recovery
102.3%
Dilution Linearity
96.5%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures total MMP-9
Assay format is 96 wells
Quality Controls are human serum based
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Bone and cartilage metabolism, Cardiovascular disease, Extracellular matrix, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Others, Pulmonary diseases
Summary
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases, responsible for the integrity of the basement membrane (BM) via degradation of extracellular matrix and BM components. MMPs are presented in various types of cells, including cardiomyocytes, cerebral neurons, hepatocytes, and many others, thus influencing various processes.
Increased matrix metalloprotease 9 (MMP9), also called Gelatinase B, after myocardial infarction (MI) exacerbates ischemia-induced chronic heart failure (CHF). It is also upregulated in the diabetic heart, and ablation of MMP9 decreases infarct size in the non-diabetic myocardial infarction heart.3 There is also strong evidence that the increased levels of MMP-9 are associated with many inflammatory conditions, such as coronary artery disease, COPD, arthritis, and metabolic syndrome. It was also proved that MMP-9 is associated with progression of atherosclerosis and higher risk of cardiovascular events. It was also suggested that elevated levels of MMP-9 (combined with MMP-2) are associated with highly tumorigenic cancers.
Instructions for Use (RUO)
Instructions for Use (RUO)
Product Brochure
Product description
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