Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
5 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.625 - 40 ng/ml
Limit of Detection
0.25 ng/ml
Intra-assay (Within-Run)
n = 8
CV = 2.5%
Inter-assay (Run-to-Run)
n = 8
CV = 5.8%
Spiking Recovery
99.7%
Dilution Linearity
106.8%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures total MMP-2
Assay format is 96 wells
Quality Controls are human serum based
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Bone and cartilage metabolism, Cardiovascular disease, Coronary artery disease, Extracellular matrix, Oncology
Summary
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases, responsible for the integrity of the basement membrane (BM) via degradation of extracellular matrix and BM components. MMPs are presented in various types of cells, including cardiomyocytes, cerebral neurons, hepatocytes, and many others, thus influencing various processes.
MMP-2, also called gelatinase A, is reported to play a crucial intracellular role where it cleaves several sarcomeric proteins inside cardiac myocytes during oxidative stress-induced injury.2 Elevated levels of MMP-2 (combined with MMP-9) are also associated with highly tumorigenic cancers, such as breast, gastric.
Instructions for Use (RUO)
Instructions for Use (RUO)
Product Brochure
Product description
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