Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
1 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.156 - 10 U/ml
Limit of Detection
0.04 U/ml
Intra-assay (Within-Run)
n = 8
CV = 2%
Inter-assay (Run-to-Run)
n = 8
CV = 2%
Spiking Recovery
100.8%
Dilution Linearity
102.7%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures KL-6
Assay format is 96 wells
Quality Controls are human serum based
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Immune Response, Infection and Inflammation, Oncology, Pulmonary diseases, COVID-19
Summary
Interstitial lung diseases (ILD) are a group of lung disorders characterized by interstitial lung thickening. Krebs von den Lungen-6 (KL-6) is a 200 kDa glycoprotein that is predominantly expressed by damaged alveolar type II cells, and it has been proposed as a potential biomarker of different ILD.
KL-6 has profibrotic and anti-apoptotic effects on lung fibroblasts and reflects the degree and severity of alveolar epithelial injury. KL-6 is predictive biomarker useful in the clinical management of ILD patients, particularly in patients with severe fibrotic lung disorders.
Serum KL-6 levels were also found elevated in Rheumatoid Arthritis – Associated IDL patients, and the levels positively correlated with HRCT fibrosis score.