| Cat. No. | Size |
Price |
|
|---|---|---|---|
| New RAG031R | 96 wells (1 kit) | $590 |
Sandwich ELISA
Plasma, Cell culture supernatant
50 µl/well
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
0.625 - 40 ng/ml
0.4 ng/ml
n = 5, CV = 5.5%
n = 5, CV =6.7%
120%
83%
It is intended for research use only The total assay time is less than 3.5 hours The kit measures specifically human and mouse Isthmin-1 Assay format is 96 wells Standard is a recombinant protein Components of the kit are provided ready to use, concentrated or dried
Cardiovascular disease, Energy metabolism and body weight regulation, Oncology, Renal disease
A recent study showed that Isthmin 1 is an adipokine that induces glucose uptake in human and mouse adipocytes. Isthmin 1 is secreted by mature adipocytes and triggers a signaling cascade similar to that of insulin, regulating glucose uptake while suppressing lipid accumulation. Recombinant Isthmin 1 or overexpression of Isthmin 1 causes a robust increase in GLUT4 dependent glucose uptake in cultured primary murine and immortalized human adipocytes as well as in primary human muscle cells and prevents insulin resistance and hepatic steatosis in a diet-induced obesity mouse model. Ablation of Isthmin 1 causes glucose intolerance and impaired insulin-stimulated adipocyte glucose uptake. Isthmin 1 suppresses de novo lipogenesis and increases protein synthesis in hepatocytes whereas Isthmin 1 knockdown in adipocytes reduces glucose uptake and insulin-dependent phosphorylation of protein kinase AKT. Isthmin 1 signaling is dependent on PI3K and shares downstream phosphorylation targets with insulin signaling, and does not seem to act through the insulin receptor or the insulin-like growth factor 1 receptor; it is most likely to signal through another, yet-to-be-identified, receptor tyrosine kinase. Multiple studies have focused on the functional analysis of ISM1 in several events, including angiogenesis, metabolism, organ homeostasis, immunity and cancer. In most processes, Isthmin 1 plays a role in homeostasis and in suppressing inflammation via Isthmin 1-GRP78 signaling by controlling macrophages populations and functions. Isthmin 1 is a potential biomarker for diseases such as obesity, diabetes type II, metabolic diseases and some cancers.