Type
Sandwich ELISA
Applications
Serum, Urine, Plasma
Sample Requirements
2 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.125 - 4 ng/ml
Limit of Detection
31 pg/ml
Intra-assay (Within-Run)
n = 8
CV = 5%
Inter-assay (Run-to-Run)
n = 8
CV = 7.5%
Spiking Recovery
110.0%
Dilution Linearity
86.3%
Features
It is intended for research use only
The total assay time is less than 3.5 hours
The kit measures IGFBP7
Assay format is 96 wells
Quality Controls are human serum based
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Apoptosis, Diabetology - Other Relevant Products, Oncology, Renal disease
Summary
IGFBP7 is a member of the insulin superfamily of growth promoting peptides, and is one of the most abundant and common growth factor polypeptides. IGFBPs are a class of secreted proteins that is able to interact with many ligands other than IGFs. Currently, IGFBP-7 has been used as an early diagnosis and prognostic marker for acute renal insufficiency (AKI). IGFBP-7, and TIMP-2 concentrations in the urine of children after receiving injections of contrast medium increased faster than SCr. The combination of IGFBP-7 and TIMP-2 was better than either analyte alone (the specificity was 80.0%, and the sensitivity was 81.2%). Based on the results of two studies, the urine compound of TIMP-2 and IGFBP-7 became US Food and Drug Administration (FDA)-approved biomarker for risk assessment of AKI in ICU patients.
IGFBP-7 has also been proposed as a potential prognostic biomarker in heart failure (HF). It provided prognostic information incremental to clinical variables, NT-proBNP, and hsTnT.
Instructions for Use (RUO)
Instructions for Use (RUO)
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