Type
Sandwich ELISA
Description
The human H-FABP ELISA kit is to be used for the in vitro quantitative determination of human H-FABP in serum or plasma samples. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures.
The kit is presented in a two assay format. The normal format takes about 1¼ hours. The rapid format takes about 45 minutes.
The analysis should be performed by trained laboratory professionals
Applications
Serum, Plasma
Sample Requirements
100 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
102 - 25 000 pg/ml
Limit of Detection
102 pg/ml
Features
- RUO
- the total assay time is less than 1.5 hours or 45 minutes for rapid version
- 2 x 96 wells
- calibration range 102- 25 000 pg/ml
- minimum concentration which can be measured is 102 pg/ml
Research topic
Cardiovascular disease, Energy metabolism and body weight regulation, Renal disease
Summary
Fatty acid-binding proteins (FABPs) are a class of cytoplasmic proteins that bind long chain fatty acids. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas.
Following acute myocardial infarction (AMI) the small protein H-FABP is rapidly released into the circulation. H-FABP is derived from the human FABP3 gene. Significantly elevated serum/plasma concentrations are found within 3 h after AMI which generally return to normal values within 12 to 24 h. These features make H-FABP a useful research tool for the early assessment or exclusion of AMI, and for the monitoring of a recurrent infarction. Constitutive H-FABP released from the heart after AMI is quantitatively recovered in serum/plasma. Thus assessment of H-FABP is also a very effective tool for the estimation of the infarct size. The human H-FABP kit can also be used for measurement of brain-type FABP, a marker for brain injury detection and for measurement of muscle-type cytosolic fatty acid binding protein (FABPc) in skeletal muscle.
In serum/plasma of healthy individuals approximately 1.6 ng/ml of H-FABP is present. H-FABP shows a slight increase with age.
Instructions for Use (RUO)
Instructions for Use (RUO)
Find documents for the lot
