Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
1µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
0.5 - 32 ng/ml
Limit of Detection
0.1 ng/ml
Intra-assay (Within-Run)
n = 12
CV = 6.2%
Inter-assay (Run-to-Run)
n = 8
CV = 4.1%
Spiking Recovery
88 - 94%
Dilution Linearity
93 - 103%
Features
It is intended for research use only
The total assay time is less than 3.5 hours
The kit measures Glutathione Peroxidase 3 in serum, plasma and cell cuture supernatant
Assay format is 96 wells
Components of the kit are provided ready to use, concentrated or dried
Research topic
Oxidative stress
Summary
Glutathione Peroxidase 3 is a 226 amino acid member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100 kDa homotetramer consisting of four identical 23 kDa subunits. GPX3 exhibits highest levels of mRNA expression in liver, kidney, heart and lung tissues. Correspondingly, high levels of GPX3 are associated with oxidative stress in metabolic diseases or in oncology.
Instructions for Use (RUO)
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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