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Human Glutathione Peroxidase 3 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:GPX3, Plasma Glutathione Peroxidase,
  • Species:Human
Cat. No. Size Price


RAG028R 96 wells (1 kit) $664
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

1µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

0.5 - 32 ng/ml

Limit of Detection

0.1 ng/ml

Intra-assay (Within-Run)

n = 12
CV = 6.2%

Inter-assay (Run-to-Run)

n = 8
CV = 4.1%

Spiking Recovery

88 - 94%

Dilution Linearity

93 - 103%

Summary

Features

It is intended for research use only

The total assay time is less than 3.5 hours

The kit measures Glutathione Peroxidase 3 in serum, plasma and cell cuture supernatant

Assay format is 96 wells

Components of the kit are provided ready to use, concentrated or dried

Research topic

Oxidative stress

Summary

Glutathione Peroxidase 3 is a 226 amino acid member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100 kDa homotetramer consisting of four identical 23 kDa subunits. GPX3 exhibits highest levels of mRNA expression in liver, kidney, heart and lung tissues. Correspondingly, high levels of GPX3 are associated with oxidative stress in metabolic diseases or in oncology.

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