Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
20 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
25 - 800 pg/ml
Limit of Detection
6 pg/ml
Intra-assay (Within-Run)
n = 8
CV = 4%
Inter-assay (Run-to-Run)
n = 8
CV = 6.5%
Spiking Recovery
105.7%
Dilution Linearity
93.7%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures GDF-15
Assay format is 96 wells
Quality Controls are human serum based
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Cardiovascular disease, Cytokines and chemokines and related molecules, Oncology, Reproduction
Summary
Growth differentiation factor 15 (GDF15) is a member of the TGFβ superfamily whose expression is increased in response to cellular stress and disease as well as by metformin. Identified as a new heart-derived endocrine hormone that regulates body growth, GDF15 has a local cardioprotective role, presumably due to its autocrine/paracrine properties: antioxidative, anti-inflammatory, antiapoptotic. GDF15 expression is highly induced in cardiomyocytes after ischemia/reperfusion and in the heart within hours after myocardial infarction (MI). GDF15 may be a predictive biomarker of adverse cardiac events. Available evidence also suggests that a substantial amount of GDF15 is secreted in various human cancers, such as ovarian cancer, prostate cancer, and breast cancer, among others.
Instructions for Use (RUO)
Instructions for Use (RUO)
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