Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
Serum: 5µl/well
Plasma: 25µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
7.781 - 500 pg/ml
Limit of Detection
3 pg/ml
Intra-assay (Within-Run)
n = 8
CV = 5.7%
Inter-assay (Run-to-Run)
n = 5
CV = 8.6%
Spiking Recovery
93 - 109%
Dilution Linearity
92 - 109%
Features
It is intended for research use only
The total assay time is less than 4 hours
The kit measures APRIL in serum, plasma and cell cuture supernatants
Assay format is 96 wells
Components of the kit are provided ready to use, concentrated or dried
Research topic
Apoptosis, Autoimmunity, Cytokines and chemokines and related molecules, Immunology, Oncology
Summary
APRIL is expressed by a number of myeloid-derived cell types including BM granulocytes, megakaryocytes, eosinophils and osteoclasts and by dendritic cells following exposure to IFNα, IFNγ or CD40L. APRIL expression is induced during hematopoiesis in the bone marrow. APRIL expression is not limited to cells of myeloid origin, but can also be found in epithelial cells of the gut, tonsil, breast and skin. Finally, APRIL is expressed in tumor cell lines and human cancer cells of colon, thyroid and lymphoid origin.
The B cell-stimulating molecules, BAFF (B cell activating factor) and APRIL (a proliferation-inducing ligand), are implicated in several human autoimmune diseases with autoreactive B cell involvement, including systemic lupus erythematosus (SLE), Sjögren’s syndrome (SS), IgA nephropathy (IgAN) and rheumatoid arthritis (RA). APRIL might also function in enhancing proliferation of some tumor cells, especially B-cell malignancies.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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