Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.082 - 20 ng/ml
Limit of Detection
0.08 ng/ml
Intra-assay (Within-Run)
CV <10%
Inter-assay (Run-to-Run)
CV <12%
Spiking Recovery
serum 116.1%, plasma 134%, cell culture media 141.4%
Dilution Linearity
serum 99.28%, plasma 99.48%, cell culture media 121.6%
Specificity
This ELISA antibody pair detects human ApoA1. Cross-reacts with ApoA1 from rhesus and cynomolgus macaques. Other species not determined.
Features
- RUO
- calibration range 0.082 - 20 ng/ml
- limit of detection 0.08 ng/ml
- intra-assay CV <10%
- inter-assay CV < 12%
Summary
The BioVendor ApoA ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ApoA in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human ApoA coated on a 96-well plate. Standards and samples are pipetted into the wells and ApoA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman ApoA antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ApoA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Instructions for Use (RUO)
Instructions for Use (RUO)
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