hsa-miR-93-5p Two-Tailed PRIMERs

Cat. No.	Size	Price
RDTT0000093PRI	50 rxn/ 150 rxn

Technical Data

Don't forget

For successful completion of the assay, we strongly recommend purchasing of our Two-Tailed cDNA Synthesis System and Two-Tailed qPCR Master Mix. The combination of these three products will provide you with the highest quality results.

Type

Two-Tailed RT/ PCR Primers

Shipping

Frozen. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).

Note

Below, links to independent papers developing and using Two-Tailed PCR can be found

Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
Click here for more papers citing Two-tailed PCR

Summary

Features

For research use only.
For measurements specific miRNA in biological fluids.
Components of the kit are provided ready to use.

Research topic

Cardiovascular disease, Oncology, Renal disease

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

Recent studies indicate that miRNAs function as oncogenes and tumor suppressors. Dysregulated miRNAs can promote tumorigenesis and cancer progression and even result in poor cancer prognosis. Cancer-specific miRNAs are present in extracellular body fluids, and may play an important role in crosstalk between cancer cells and surrounding normal cells.

hsa-miRNA-93 is a member of the miR-106b-25 cluster. hsa-miR-93 has been reported to be upregulated and have a promoting role in several cancer types. Increasing body of evidence indicates that the dysregulation of hsa-miR-93-5p is associated with development of multidrug resistance in various types of cancer.

miRNAs are also key regulators of many cellular events in pathogenesis of atherosclerosis. hsa-miR-93-5p is specifically associated with coronary artery disease. Several groups have reported potential utility of miRNAs as biomarkers in diagnosis and prediction of cardiovascular disease.

Moreover, hsa-miR-93-5p might help to assess pathophysiological changes associated with chronic kidney disease and potential bone-specific and vascular risks as well as therapy aspects in pacients with chronic kidney disease.

Summary References (9)

References to miR-93-5p

SUETA, Aiko, et al. Differential expression of exosomal miRNAs between breast cancer patients with and without recurrence. Oncotarget, 2017, 8.41: 69934.
LI, Nana, et al. MiR-106b and miR-93 regulate cell progression by suppression of PTEN via PI3K/Akt pathway in breast cancer. Cell Death & Disease, 2017, 8.5: e2796.
WANG, Shi Jun, et al. MicroRNA 93 5p increases multidrug resistance in human colorectal carcinoma cells by downregulating cyclin dependent kinase inhibitor 1A gene expression. Oncology Letters, 2017, 13.2: 722-730.
GAMBARI, Roberto, et al. Targeting oncomiRNAs and mimicking tumor suppressor miRNAs: Νew trends in the development of miRNA therapeutic strategies in oncology. International journal of oncology, 2016, 49.1: 5-32.
HE, Yu; YU, Bo. MicroRNA 93 promotes cell proliferation by directly targeting P21 in osteosarcoma cells. Experimental and Therapeutic Medicine, 2017, 13.5: 2003-2011.
OHTA, Katsuya, et al. MicroRNA-93 activates c-Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A. Oncotarget, 2015, 6.5: 3211.
CHEN, Rui, et al. MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide. Biology open, 2016, 5.6: 669-677.
JOHN, F. O., et al. miRNA-93-5p and other miRNAs as predictors of coronary artery disease and STEMI. International journal of cardiology, 2016, 224: 310-316.
ULBING, M., et al. MicroRNAs 223-3p and 93-5p in patients with chronic kidney disease before and after renal transplantation. Bone, 2017, 95: 115-123.

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Product Datasheet (RUO)

TT-PCR manual

Safety Information (RUO)

MSDS (RUO)

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Product description