hsa-miR-486-5p Two-Tailed PRIMERs

Cat. No.	Size	Price
RDTT0002177PRI	50 rxn/ 150 rxn

Technical Data

Don't forget

For successful completion of the assay, we strongly recommend purchasing of our Two-Tailed cDNA Synthesis System and Two-Tailed qPCR Master Mix. The combination of these three products will provide you with the highest quality results.

Type

Two-Tailed RT/ PCR Primers

Shipping

Frozen. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).

Note

Below, links to independent papers developing and using Two-Tailed PCR can be found

Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
Click here for more papers citing Two-tailed PCR

Summary

Features

For research use only.
For measurements specific miRNA in biological fluids.
Components of the kit are provided ready to use.

Research topic

Oncology

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

hsa-miR-486-5p is located on the chromosome 8, in the region which contains potential tumor-suppressor genes and is often deleted in various types of tumors. hsa-miR-486-5p gene was first cloned from the fetal liver. Reduced miR‑486-5p expression is a frequent molecular event in human malignancies.

Deregulation of cancer-related miR-486-5p is a common event in both benign and malignant human breast tumors. miR-486-5p is one of the most downregulated miRNAs in lung tumor tissues. Its decreased level in circulation indicates lung cancer progression and metastasis. Thus, serum miR-486-5p has a great potential to serve as a marker in clinical diagnosis of lung cancers. Several studies have also shown aberrant miR-486-5p expression and its prognostic value in digestive system cancers. miR-486-5p may become a novel target in treatment of patients with gastric cancers. Other cancer types associated with deregulated miR-486-5p levels are hepatocellular carcinomas and prostate cancer.

Summary References (9)

References to miR-486-5p

WANG, J., et al. Downregulation of miR-486-5p contributes to tumor progression and metastasis by targeting protumorigenic ARHGAP5 in lung cancer. Oncogene, 2014, 33.9: 1181.
ZHU, Jianjie, et al. Expression profile analysis of microRNAs and downregulated miR-486-5p and miR-30a-5p in non-small cell lung cancer. Oncology reports, 2015, 34.4: 1779-1786.
TAHIRI, Andliena, et al. Deregulation of cancer-related miRNAs is a common event in both benign and malignant human breast tumors. Carcinogenesis, 2013, 35.1: 76-85.
LI, Ying; LIANG, Li; ZHANG, Chun-yang. Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis. Analytical chemistry, 2013, 85.23: 11174-11179.
TIAN, Fei, et al. Aberrant miR-181b-5p and miR-486-5p expression in serum and tissue of non-small cell lung cancer. Gene, 2016, 591.2: 338-343.
CHEN, Hui, et al. Expression and prognostic value of miR-486-5p in patients with gastric adenocarcinoma. PLoS One, 2015, 10.3: e0119384.
REN, Chuanli, et al. miR-486-5p expression pattern in esophageal squamous cell carcinoma, gastric cancer and its prognostic value. Oncotarget, 2016, 7.13: 15840.
HUANG, Xin‐Ping, et al. MicroRNA‐486‐5p, which is downregulated in hepatocellular carcinoma, suppresses tumor growth by targeting PIK3R1. The FEBS journal, 2015, 282.3: 579-594.
YANG, Yang, et al. The miR-486-5p plays a causative role in prostate cancer through negative regulation of multiple tumor suppressor pathways. Oncotarget, 2017, 8.42: 72835.

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TT-PCR manual

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