Type
Two-Tailed RT/ PCR Primers
Shipping
Frozen. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).
Note
Below, links to independent papers developing and using Two-Tailed PCR can be found
- Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
- Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
- Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
- Click here for more papers citing Two-tailed PCR
Features
- For research use only.
- For measurements specific miRNA in biological fluids.
- Components of the kit are provided ready to use.
Research topic
Oncology, Reproduction, Stroke
Summary
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
miR-210 represents the major hypoxia-inducible miRNA also known as hypoxamir, which is ubiquitously expressed in a broad range of cells. Emerging evidence has demonstrated that induction of miR-210-3p is a consistent feature of the hypoxic response in both normal and malignant cells. miR-210 has been associated with a variety of functionally important targets involved in cancer, cell cycle regulation, cell survival, differentiation, metabolism as well as in angiogenesis.
Increase miR-210 plasma level has been reported in the context of various cancers and other hypoxia-induced pathological conditions. It has been described that hypoxic cancer cells release exosomes with high levels of miR-210. miR-210 is frequently elevated in osteosarcoma, breast, lung, head and neck, and pancreatic cancer, or glioblastoma. In this regard, it was described that circulating miR-210 was significantly elevated in patients with clear cell or conventional renal cell carcinoma (ccRCC) compared with healthy controls. More recently, the amount of miR-210 has been analyzed in cell-free urine as a tool for liquid biopsy in ccRCC and the level of urinary cell-free miR-210 was significantly higher in patients with ccRCC than in control subjects. Furthermore, serum miR-210 was up-regulated in patients with bladder cancer.
Aberrant expression of miR-210 has also been reported in other diseases than cancer. Blood miRNA-210 was significantly decreased in stroke patients and its level was significantly higher in stroke patients with good outcome than that in patients with poor outcome. Moreover, it has been demonstrated that overexpression of miR-210 mediates mitochondrial dysfunction in the placenta of women with pre-eclampsia. miR-210-3p is supposed to be involved in endometriotic cell proliferation and the results show that increased levels of miR-210-3p in endometriotic lesions contribute to endometriosis progression. The potential role of miR-210 in spermatogenesis was also characterized. The results indicated that elevated expression of seminal plasma miR‐210-3p was an independent predictor of impaired spermatogenic function in varicocele patients.
