hsa-miR-191-5p Two-Tailed PRIMERs

Cat. No.	Size	Price
RDTT0000440PRI	50 rxn/ 150 rxn

Technical Data

Don't forget

For successful completion of the assay, we strongly recommend purchasing of our Two-Tailed cDNA Synthesis System and Two-Tailed qPCR Master Mix. The combination of these three products will provide you with the highest quality results.

Type

Two-Tailed RT/ PCR Primers

Applications

COVID-19

Shipping

Frozen. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).

Note

Below, links to independent papers developing and using Two-Tailed PCR can be found

Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
Click here for more papers citing Two-tailed PCR

Summary

Features

For research use only.
For measurements specific miRNA in biological fluids.
Components of the kit are provided ready to use.

Research topic

Oncology

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length, that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

miR-191 is expressed as a part of the miR-191/425 cluster on human chromosome 3.

miR-191 is developmentally regulated; significantly higher levels of miR-191 were found in human prefrontal cortex specimens from individuals older than 41 years compared to samples from individuals under 15 years. It was suggested that miR-191-mediated downregulation of a brain-derived neurotrophic factor may contribute to cortical development.

miR-191 was found to be significantly downregulated during terminal erythroid differentiation while its overexpression inhibited erythroid enucleation and chromatin condensation, suggesting that miR-191 plays a crucial role in erythropoiesis. miR-191 was also found to be differentially expressed in monocytes vs. monocyte-derived dendritic cells.

Changes in miR-191 expression have been reported in various malignancies such as breast, colon, lung, liver, prostate, pancreas, stomach, and ovarian cancer, pituitary adenoma, esophageal squamous carcinoma, oral squamous carcinoma, osteosarcoma, bladder, anaplastic large cell lymphoma, acute myeloid leukemia, severe medulloblastoma, retinoblastoma, thyroid follicular tumor, male breast cancer and melanoma.

Besides cancer, aberrant expression and functional abnormalities of miR-191 have been reported in a variety of other diseases, too. miR-191 may be a diagnostic biomarker that could help to distinguish Crohn’s disease from ulcerative colitis. Similarly, miR-191 was shown to be a potential biomarker for pulmonary hypertension, enabling its early detection and also, indicating the disease severity. miR-191 was also found to be induced in nephrotic syndrome in children, which makes it a potential diagnostic marker for this disease.

Summary References (9)

References to miR-191-5p

Kiezun, Adam, et al. "miRviewer: a multispecies microRNA homologous viewer." BMC research notes 5.1 (2012): 92.
Griffiths-Jones, Sam, et al. "miRBase: microRNA sequences, targets and gene nomenclature." Nucleic acids research34.suppl_1 (2006): D140-D144.
Mellios, Nikolaos, et al. "A set of differentially expressed miRNAs, including miR-30a-5p, act as post-transcriptional inhibitors of BDNF in prefrontal cortex." Human molecular genetics 17.19 (2008): 3030-3042.
Zhang, Lingbo, et al. "miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1." Genes & development 25.2 (2011): 119-124.
Cekaite, L., T. Clancy, and M. Sioud. "Increased miR-21 expression during human monocyte differentiation into DCs." Frontiers in bioscience (Elite edition) 2 (2010): 818-828.
Nagpal, Neha, and Ritu Kulshreshtha. "miR-191: an emerging player in disease biology." Frontiers in genetics 5 (2014): 99.
Lin, Jingmei, et al. "MicroRNA expression patterns in indeterminate inflammatory bowel disease." Modern Pathology26.1 (2013): 148.
Wei, Chuanyu, et al. "Circulating miRNAs as potential marker for pulmonary hypertension." PloS one 8.5 (2013): e64396.
Luo, Yang, et al. "Increased serum and urinary microRNAs in children with idiopathic nephrotic syndrome." Clinical chemistry59.4 (2013): 658-666.

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Product Datasheet (RUO)

TT-PCR manual

Safety Information (RUO)

MSDS (RUO)

Product Brochure

Product description