hsa-miR-16-5p Two-Tailed PRIMERs

Cat. No.	Size	Price
RDTT0000069PRI	50 rxn/ 150 rxn

Technical Data

Don't forget

For successful completion of the assay, we strongly recommend purchasing of our Two-Tailed cDNA Synthesis System and Two-Tailed qPCR Master Mix. The combination of these three products will provide you with the highest quality results.

Type

Two-Tailed RT/ PCR Primers

Shipping

Frozen. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).

Note

Below, links to independent papers developing and using Two-Tailed PCR can be found

Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
Click here for more papers citing Two-tailed PCR

Summary

Features

For research use only.
For measurements specific miRNA in biological fluids.
Components of the kit are provided ready to use.

Research topic

Autoimmunity, Immune Response, Infection and Inflammation, Oncology

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases. miR-16, located on the 13q14 chromosome, was initially found to be related to chronic lymphocytic leukemia. Studies reveal that miR-16 is down-regulated and plays a tumor suppressive role by affecting cell proliferation, cell cycle, invasion and apoptosis in different types of malignancies, such as breast cancer, lung cancer, ovarian cancer, osteosarcoma and prostate cancer. In contrast, other studies have reported that miR-16 is up-regulated in renal cell carcinoma and espohageal squamous cell carcinoma where it acts as an oncogene by inducing cellular proliferation and migration and reducing apoptosis. The level of miR-16-5p was significantly decreased in plasma of systemic lupus erythematosus patients compared with healthy controls and also in serum of patients with rheumatoid arthritis compared to healthy controls.

Summary References (10)

References to miR-16-5p

Humplikova, Lenka, et al. "Expression of miR-15a and miR-16-1 in patients with chronic lymphocytic leukemia." Biomedical Papers 157.4 (2013): 284-293.
Mobarra, Naser, et al. "Overexpression of microRNA-16 declines cellular growth, proliferation and induces apoptosis in human breast cancer cells." In Vitro Cellular & Developmental Biology-Animal 51.6 (2015): 604-611.
Zhu, Yi, et al. "MiR-16 induced the suppression of cell apoptosis while promote proliferation in esophageal squamous cell carcinoma." Cellular Physiology and Biochemistry 33.5 (2014): 1340-1348.
Fu, Xiafei, et al. "MicroRNA-16 Promotes Ovarian Granulosa Cell Proliferation and Suppresses Apoptosis Through Targeting PDCD4 in Polycystic Ovarian Syndrome." Cellular Physiology and Biochemistry 48.2 (2018): 670-682.
Bonci, Désirée, et al. "The miR-15a–miR-16-1 cluster controls prostate cancer by targeting multiple oncogenic activities." Nature medicine 14.11 (2008): 1271.
Jiao, Z. H., J. D. Wang, and X. J. Wang. "MicroRNA-16 suppressed the invasion and migration of osteosarcoma by directly inhibiting RAB23." European review for medical and pharmacological sciences 22.9 (2018): 2598-2605.
Bandi, Nora, et al. "miR-15a and miR-16 are implicated in cell cycle regulation in a Rb-dependent manner and are frequently deleted or down-regulated in non–small cell lung cancer." Cancer research 69.13 (2009): 5553-5559.
Chen, Duqun, et al. "Upregulated microRNA-16 as an oncogene in renal cell carcinoma." Molecular medicine reports 12.1 (2015): 1399-1404.
Zhang, Huidi, et al. "B cell-related circulating microRNAs with the potential value of biomarkers in the differential diagnosis, and distinguishment between the disease activity and lupus nephritis for SLE." Frontiers in immunology 9 (2018): 1473.
Dunaeva, Marina, et al. "Circulating serum miR‐223‐3p and miR‐16‐5p as possible biomarkers of early rheumatoid arthritis." Clinical & Experimental Immunology (2018).

Docs

Product Datasheet (RUO)

TT-PCR manual

Safety Information (RUO)

MSDS (RUO)

Product Brochure

Product description