Type
Active protein
Description
Total 99 AA. MW: 10.8 kDa (monomer), protein active as dimer
Amino Acid Sequence
PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
Source
E. coli
Purity
Purity as determined by densitometric image analysis: >90%
SDS-PAGE Gel
14% SDS-PAGE separation of Human HIV-1 Protease
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 2.5 μg/lane
Formulation
20 mM sodium acetate, 200 mM NaCl, 10% glycerol, 0.05% 2-mercaptoethanol, 1 mM EDTA, pH 5.0
Reconstitution
Defrost at ambient temperature.
Applications
Kinetic studies, Inhibitor screening, Crystallography
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store protein at -80°C. Protein remains stable until the expiry date when stored at -80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
SDS PAGE to determine purity of the protein.
Active site titration by tightly binding inhibitor.
Note
Km = 15.1 μM Kcat = 30 s-1 Kcat /Km = 1981 mM-1 s-1 with peptide substrate KARVF(NO2 )VRKA (F(NO2 ) … p-nitrophenylalanine)