Type
Sandwich ELISA, AChE-labelled antibody
Applications
Plasma-EDTA
Sample Requirements
10 µl/well
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at -20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Calibration Range
1.96–250 pg/ml
Limit of Detection
The Limit of Determination, calculated as the concentration of unacylated ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.6 pg/ml and 0.8 pg/ml for long and short first incubation period, respectively.
Intra-assay (Within-Run)
CV = 8.2% (20h at +4°C)
CV = 9.2% (3h at RT)
Inter-assay (Run-to-Run)
CV = 8.6% (20h at +4°C)
CV = 9.5% (3h at RT)
Spiking Recovery
101.5% (20h at +4°C)
99.8% (3h at RT)
Dilution Linearity
98,90%
Features
- The kit measures plasma unacylated ghrelin.
- Quality controls are rat-plasma based.
Research topic
Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Animal studies
Summary
Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
Product Brochure
Ghrelin and Obestatin: Twin proteins encoded by the one gene
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