Type
Sandwich ELISA, AChE-labelled antibody
Description
This Enzyme Immunometric Assay (EIA) is based on a doubleantibody sandwich technique.
Applications
Plasma
Sample Requirements
100 µl/well
Shipping
On dry ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at -20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Calibration Range
250 - 2 pg/ml
Limit of Detection
The limit of detection, calculated as the concentration of Acylated Ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.8 pg/mL. Due to the minimal plasma dilution (1:5), the limit of detection in the samples is less than 5 pg/mL.
Intra-assay (Within-Run)
CV < 12% (20h at +4°C)
,CV < 7% (3h at RT)
Inter-assay (Run-to-Run)
CV < 11.5 % (20h at +4°C)
CV < 8.5% (3h at RT)
Spiking Recovery
99.5% (20h at +4°C)
98.6% (3h at RT)
Features
- RUO
- calibration range 2-250 pg/ml
- QC has been prepared by spiking Acylated Ghrelin (mouse,rat) peptide in Dilution Buffer
- blood samples are collected in tubes containing EDTA
Research topic
Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Animal studies
Summary
Ghrelin discovered in 1999, is fast becoming an endokrinology target of the millennium. Ghrelin, identifi ed in rat stomach as an endogenous ligand for the GH secretagogue receptor, is mainly produced in stomach, but has been demonstrated in many other organs [4, 5]. In addition to GH-releasing properties and its orexant action, Ghrelin could act as an hormone having effects on gastric motility (similarity with the peptide hormone motilin), acidic secretion, cardiovascular action, antiproliferative effects, pancreatic and glucose metabolism function, sleep [6, 7, 8]...
Ghrelin gene raises to mRNA prepro-ghrelin of 117 amino acids. This precursor is processed into Ghrelin, 28 amino acids (human). Before being secreted, this peptide is octanoylated at Ser 3 by GOAT (Ghrelin Octanoyl Acyl Transferase). This step is Essentials for biological activity making GOAT a perfect target for drugs in feeding behaviour. Interestingly, the potential therapeutic importance of this hormone is not restricted to regulation of food intake [9] but also in cachexia (related to cancer treatment, anorexia nervosa or ischemia) [10] gastrin motility and may be involved in osteoporosis, somatopause, infertility and ovulation induction, neurological disorders (Alcoholism, Post Traumatic Stress disorders...) [11] and cardiovascular diseases.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
Product Brochure
Ghrelin and Obestatin: Twin proteins encoded by the one gene
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