Type
Sandwich ELISA, Biotin-labelled antibody
Description
In GADAb ELISA, GADAb in patients’ sera, calibrators and controls are allowed to interact with GAD65 coated onto ELISA plate wells.
Applications
Serum
Sample Requirements
25 ul
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
5–2000 U/ml
Limit of Detection
0.57 U/ml
Intra-assay (Within-Run)
CV = 6.4%
Inter-assay (Run-to-Run)
CV = 5.8%
Specificity
In the DASP 2005 study the GADAb ELISA kit achieved 98% (n=100) specificity and 92% (n=50) sensitivity.