Type
Recombinant protein
Source
E. coli
Purity
˃ 95 % by SDS-PAGE
Endotoxin
< 1.0 EU/µg
Formulation
White lyophilized (freeze-dried) powder, lyophilized from 20 mM potassium phosphate, 750 mM sodium chloride, pH 7.5.
Reconstitution
Reconstitute to 1.0 mg/mL by addition of 50 ul of water to each vial. This solution can then be further diluted into other aqueous solutions. Store reconstituted sample at 2 °C to 8 °C for 1 week.
Storage/Expiration
Store the lyophilized protein at 4 °C. Lyophilized protein remains stable until the expiry date when stored at 4 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 2 °C to 8 °C for 1 week.
Quality Control Test
UV spectrophotometry at 280 nm to determine quantity of the protein.
Bioactivity.
SDS-PAGE to determine purity of the protein.
Endotoxin level determination.
Note
This product is intended for research use only.